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- W1602291923 abstract "Changes of endocytotic activities during the cell cycle of the cellular slime mould Dictyostelium discoideum Ax-2 were examined using the temperature-shift method for inducing synchronous growth. The activity of fluid-phase pinocytosis (FPP) was altered Ca2+ -dependently and stimulated by EGTA. On the other hand, pinocytosis was greatly enhanced by addition of Bacteriological-peptone(BP) to the growth medium for Ax-2 cells, irrespective of the extracellular Ca2+ -concentration. The maximal pinocytotic activity was attained in the presence of EGTA plus BP, the effects of the two substances being additive. The FPP activity was found to be high in cells in and just after the S phase, when the BP-induced fraction of pinocytosis was rather low. Thus the total activity for pinocytosis in the growth medium remained almost constant throughout the cell cycle, indicating that the rate of nutrient uptake through pinocytosis was not a limiting factor for cell cycle regulation. The change of phagocytotic activity during the cell cycle was somewhat similar to that of the FPP activity. Possible mechanisms of such cell-cycle related changes are discussed in relation to cytoskeletal proteins in the cell cortex. Some properties of BP-induced pinocytosis are also described." @default.
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- W1602291923 date "1988-02-01" @default.
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- W1602291923 title "Changes of Endocytotic Activities during the Cell Cycle of Dictyostelium Cells. (Dictyostelium discoideum/pinocytosis/phagocytosis/cell cycle/cytoskeleton)" @default.
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- W1602291923 doi "https://doi.org/10.1111/j.1440-169x.1988.00015.x" @default.
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