Matches in SemOpenAlex for { <https://semopenalex.org/work/W1602614663> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1602614663 endingPage "3631" @default.
- W1602614663 startingPage "3626" @default.
- W1602614663 abstract "When isolated human fibroblast lysosomes are incubated with 4 microM [32P]phosphate at pH 7.0, orthophosphate is transported into lysosomes and is rapidly incorporated into low and high molecular weight products. We have characterized the high molecular weight (HMW) lysosomal material into which [32P]phosphate is incorporated and have found it to consist of long chains of inorganic polyphosphate based on the following observations. 1) greater than 97% of HMW 32P-lysosomal material is converted to [32P]orthophosphate when incubated with 1 N HCl for 20 min at 100 degrees C. 2) Incubation of HMW 32P-lysosomal material at pH 7.0 and 65 degrees C for 96 h results in the formation of [32P]trimetaphosphate, which is known to be produced only from linear chains of polyphosphate under these conditions. 3) HMW 32P-lysosomal material is resistant to degradation by proteinase K, ribonuclease, and deoxyribonuclease and extracts into the aqueous phase during phenol/chloroform extractions. 4) HMW 32P-lysosomal material displays heterogeneous mobility on polyacrylamide gels with most chains ranging in length from 100 to at least 600 phosphate residues. 5) HMW 32P-lysosomal material is partially hydrolyzed under alkaline conditions to yield a continuous ladder of polyphosphate species differing by one or several residues in length on polyacrylamide gels." @default.
- W1602614663 created "2016-06-24" @default.
- W1602614663 creator A5057956524 @default.
- W1602614663 creator A5064166607 @default.
- W1602614663 date "1992-02-01" @default.
- W1602614663 modified "2023-10-01" @default.
- W1602614663 title "Incorporation of [32P]orthophosphate into long chains of inorganic polyphosphate within lysosomes of human fibroblasts." @default.
- W1602614663 cites W1496287649 @default.
- W1602614663 cites W1501401655 @default.
- W1602614663 cites W1505146170 @default.
- W1602614663 cites W1509440618 @default.
- W1602614663 cites W1552737826 @default.
- W1602614663 cites W1561924747 @default.
- W1602614663 cites W1569876389 @default.
- W1602614663 cites W1570863898 @default.
- W1602614663 cites W1575962485 @default.
- W1602614663 cites W1577607250 @default.
- W1602614663 cites W1583400983 @default.
- W1602614663 cites W1583898985 @default.
- W1602614663 cites W1971179476 @default.
- W1602614663 cites W1976731052 @default.
- W1602614663 cites W1980722021 @default.
- W1602614663 cites W1984505360 @default.
- W1602614663 cites W1989492043 @default.
- W1602614663 cites W2007886547 @default.
- W1602614663 cites W2012033813 @default.
- W1602614663 cites W2028456506 @default.
- W1602614663 cites W2038959364 @default.
- W1602614663 cites W2043128912 @default.
- W1602614663 cites W2054223942 @default.
- W1602614663 cites W2064076848 @default.
- W1602614663 cites W2066444129 @default.
- W1602614663 cites W2075210003 @default.
- W1602614663 cites W2079360060 @default.
- W1602614663 cites W2081621607 @default.
- W1602614663 cites W2131557001 @default.
- W1602614663 cites W2145634359 @default.
- W1602614663 doi "https://doi.org/10.1016/s0021-9258(19)50570-1" @default.
- W1602614663 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1740414" @default.
- W1602614663 hasPublicationYear "1992" @default.
- W1602614663 type Work @default.
- W1602614663 sameAs 1602614663 @default.
- W1602614663 citedByCount "70" @default.
- W1602614663 countsByYear W16026146632012 @default.
- W1602614663 countsByYear W16026146632013 @default.
- W1602614663 countsByYear W16026146632014 @default.
- W1602614663 countsByYear W16026146632015 @default.
- W1602614663 countsByYear W16026146632016 @default.
- W1602614663 countsByYear W16026146632017 @default.
- W1602614663 countsByYear W16026146632018 @default.
- W1602614663 countsByYear W16026146632019 @default.
- W1602614663 countsByYear W16026146632020 @default.
- W1602614663 countsByYear W16026146632021 @default.
- W1602614663 countsByYear W16026146632022 @default.
- W1602614663 crossrefType "journal-article" @default.
- W1602614663 hasAuthorship W1602614663A5057956524 @default.
- W1602614663 hasAuthorship W1602614663A5064166607 @default.
- W1602614663 hasBestOaLocation W16026146631 @default.
- W1602614663 hasConcept C12554922 @default.
- W1602614663 hasConcept C185592680 @default.
- W1602614663 hasConcept C2777132085 @default.
- W1602614663 hasConcept C2778038992 @default.
- W1602614663 hasConcept C55493867 @default.
- W1602614663 hasConcept C86803240 @default.
- W1602614663 hasConceptScore W1602614663C12554922 @default.
- W1602614663 hasConceptScore W1602614663C185592680 @default.
- W1602614663 hasConceptScore W1602614663C2777132085 @default.
- W1602614663 hasConceptScore W1602614663C2778038992 @default.
- W1602614663 hasConceptScore W1602614663C55493867 @default.
- W1602614663 hasConceptScore W1602614663C86803240 @default.
- W1602614663 hasIssue "6" @default.
- W1602614663 hasLocation W16026146631 @default.
- W1602614663 hasOpenAccess W1602614663 @default.
- W1602614663 hasPrimaryLocation W16026146631 @default.
- W1602614663 hasRelatedWork W1963686347 @default.
- W1602614663 hasRelatedWork W2004605658 @default.
- W1602614663 hasRelatedWork W2056621670 @default.
- W1602614663 hasRelatedWork W2060278151 @default.
- W1602614663 hasRelatedWork W2061573865 @default.
- W1602614663 hasRelatedWork W2086350928 @default.
- W1602614663 hasRelatedWork W2117626828 @default.
- W1602614663 hasRelatedWork W2147096423 @default.
- W1602614663 hasRelatedWork W2566986652 @default.
- W1602614663 hasRelatedWork W4307689867 @default.
- W1602614663 hasVolume "267" @default.
- W1602614663 isParatext "false" @default.
- W1602614663 isRetracted "false" @default.
- W1602614663 magId "1602614663" @default.
- W1602614663 workType "article" @default.