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- W1603048142 abstract "We describe the purification and properties of Dp-1, a bacteriophage isolated from Diplococcus pneumoniae. The phage was sensitive to the organic solvents deoxycholate and Sarkosyl, and its infectivity was reduced by treatment with phospholipase C. Electron microscopy indicated the presence of a double-layered coat around the phage particles. Purified phage preparations contained lipid amounting to about 8.5% of the dry weight of the phage, and thin-layer chromatography resolved the lipids into four components. The phage had a buoyant density in CsCl of 1.47 g/cm3, and a sedimentation constant in 0.1 M NaCl of 313S. Analysis in acrylamide gel electrophoresis indicated the presence of three major proteins. Dp-1 DNA shows a density of 1.681 g/cm3. Neutralizing antisera against the phage have a low potency (K less than 120/min)." @default.
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- W1603048142 date "1977-10-01" @default.
- W1603048142 modified "2023-10-17" @default.
- W1603048142 title "Properties of diplophage: a lipid-containing bacteriophage" @default.
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- W1603048142 doi "https://doi.org/10.1128/jvi.24.1.201-210.1977" @default.
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