FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1603076588> ?p ?o ?g. }

• W1603076588 endingPage "707" @default.
• W1603076588 startingPage "704" @default.
• W1603076588 abstract "The viability of Giardia muris cysts was studied with the fluorogenic dyes fluorescein diacetate (FDA) and propidium iodide (PI). G. muris cysts were seen to fluoresce intensely green with FDA at an excitation wavelength of 450 to 490 nm. Cysts stained with PI fluoresced bright orange at an excitation wavelength of 450 to 490 nm and bright red at 545 to 546 nm. Examination of isolated G. muris cyst preparations stained with FDA-PI revealed that greater than 85% of the cysts stained green with FDA and less than 15% stained orange-red with PI. Using the mouse model for giardiasis, we inoculated FDA- or PI-stained cysts into neonatal mice. Feces were examined at days 3, 5, 8, and 11 postinoculation for the presence of cysts. Using 1,000 FDA-stained cysts as the inoculum, we detected cysts at days 5, 8, and 11 postinoculation in 19 of 19 mice, whereas a 50-fold greater dose of cysts produced infection in 27 of 27 mice at day 3 as well as at days 5, 8, and 11 postinoculation. Inoculation of mice with either 5,000 or 50,000 PI-stained G. muris cysts did not produce infection in any of the animals. Necropsy of mice infected with FDA-stained cysts showed trophozoites within the intestines. No trophozoites were detected within animals inoculated with PI-stained cysts. These results demonstrate that FDA-positive cysts are viable, as determined by infectivity, while PI-positive cysts are nonviable and incapable of producing G. muris infections in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W1603076588 created "2016-06-24" @default.
• W1603076588 creator A5081337968 @default.
• W1603076588 creator A5082358736 @default.
• W1603076588 date "1987-04-01" @default.
• W1603076588 modified "2023-10-14" @default.
• W1603076588 title "A new method to determine Giardia cyst viability: correlation of fluorescein diacetate and propidium iodide staining with animal infectivity" @default.
• W1603076588 cites W1223843341 @default.
• W1603076588 cites W1573893721 @default.
• W1603076588 cites W1980181948 @default.
• W1603076588 cites W1987450033 @default.
• W1603076588 cites W2020803551 @default.
• W1603076588 cites W2038801464 @default.
• W1603076588 cites W2039003569 @default.
• W1603076588 cites W2046794481 @default.
• W1603076588 cites W2048078327 @default.
• W1603076588 cites W2061185721 @default.
• W1603076588 cites W2080055578 @default.
• W1603076588 cites W2082240063 @default.
• W1603076588 cites W2083589538 @default.
• W1603076588 cites W2092839998 @default.
• W1603076588 cites W2120435191 @default.
• W1603076588 cites W2133163037 @default.
• W1603076588 cites W2158060338 @default.
• W1603076588 cites W2169790726 @default.
• W1603076588 cites W2169966243 @default.
• W1603076588 cites W2190330961 @default.
• W1603076588 cites W36201129 @default.
• W1603076588 cites W65207546 @default.
• W1603076588 doi "https://doi.org/10.1128/aem.53.4.704-707.1987" @default.
• W1603076588 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/203740" @default.
• W1603076588 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2437857" @default.
• W1603076588 hasPublicationYear "1987" @default.
• W1603076588 type Work @default.
• W1603076588 sameAs 1603076588 @default.
• W1603076588 citedByCount "106" @default.
• W1603076588 countsByYear W16030765882012 @default.
• W1603076588 countsByYear W16030765882013 @default.
• W1603076588 countsByYear W16030765882014 @default.
• W1603076588 countsByYear W16030765882015 @default.
• W1603076588 countsByYear W16030765882018 @default.
• W1603076588 countsByYear W16030765882020 @default.
• W1603076588 countsByYear W16030765882021 @default.
• W1603076588 countsByYear W16030765882022 @default.
• W1603076588 countsByYear W16030765882023 @default.
• W1603076588 crossrefType "journal-article" @default.
• W1603076588 hasAuthorship W1603076588A5081337968 @default.
• W1603076588 hasAuthorship W1603076588A5082358736 @default.
• W1603076588 hasBestOaLocation W16030765881 @default.
• W1603076588 hasConcept C106358424 @default.
• W1603076588 hasConcept C121332964 @default.
• W1603076588 hasConcept C142724271 @default.
• W1603076588 hasConcept C150903083 @default.
• W1603076588 hasConcept C153911025 @default.
• W1603076588 hasConcept C159047783 @default.
• W1603076588 hasConcept C180032290 @default.
• W1603076588 hasConcept C190283241 @default.
• W1603076588 hasConcept C203014093 @default.
• W1603076588 hasConcept C207001950 @default.
• W1603076588 hasConcept C2522874641 @default.
• W1603076588 hasConcept C26291073 @default.
• W1603076588 hasConcept C2775934118 @default.
• W1603076588 hasConcept C2778015030 @default.
• W1603076588 hasConcept C2780597408 @default.
• W1603076588 hasConcept C31573885 @default.
• W1603076588 hasConcept C54355233 @default.
• W1603076588 hasConcept C55493867 @default.
• W1603076588 hasConcept C62520636 @default.
• W1603076588 hasConcept C71924100 @default.
• W1603076588 hasConcept C74864618 @default.
• W1603076588 hasConcept C86803240 @default.
• W1603076588 hasConcept C89423630 @default.
• W1603076588 hasConcept C91881484 @default.
• W1603076588 hasConceptScore W1603076588C106358424 @default.
• W1603076588 hasConceptScore W1603076588C121332964 @default.
• W1603076588 hasConceptScore W1603076588C142724271 @default.
• W1603076588 hasConceptScore W1603076588C150903083 @default.
• W1603076588 hasConceptScore W1603076588C153911025 @default.
• W1603076588 hasConceptScore W1603076588C159047783 @default.
• W1603076588 hasConceptScore W1603076588C180032290 @default.
• W1603076588 hasConceptScore W1603076588C190283241 @default.
• W1603076588 hasConceptScore W1603076588C203014093 @default.
• W1603076588 hasConceptScore W1603076588C207001950 @default.
• W1603076588 hasConceptScore W1603076588C2522874641 @default.
• W1603076588 hasConceptScore W1603076588C26291073 @default.
• W1603076588 hasConceptScore W1603076588C2775934118 @default.
• W1603076588 hasConceptScore W1603076588C2778015030 @default.
• W1603076588 hasConceptScore W1603076588C2780597408 @default.
• W1603076588 hasConceptScore W1603076588C31573885 @default.
• W1603076588 hasConceptScore W1603076588C54355233 @default.
• W1603076588 hasConceptScore W1603076588C55493867 @default.
• W1603076588 hasConceptScore W1603076588C62520636 @default.
• W1603076588 hasConceptScore W1603076588C71924100 @default.
• W1603076588 hasConceptScore W1603076588C74864618 @default.
• W1603076588 hasConceptScore W1603076588C86803240 @default.
• W1603076588 hasConceptScore W1603076588C89423630 @default.
• W1603076588 hasConceptScore W1603076588C91881484 @default.
• W1603076588 hasIssue "4" @default.

• next