FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1604367031> ?p ?o ?g. }

• W1604367031 endingPage "19410" @default.
• W1604367031 startingPage "19403" @default.
• W1604367031 abstract "Microspectrofluorometry (fura-2) was combined with the whole-cell patch-clamp technique to study bradykinin-activated calcium (Ca2+) influx in single control or v-Ki-ras-transformed NIH/3T3 (DT) fibroblasts. Application of bradykinin on DT cells, but not on control NIH/3T3 cells, evoked cytosolic Ca2+ oscillations in the presence of extracellular Ca2+, but not in the absence of external Ca2+. This effect of zero external Ca2+ concentration could be mimicked by holding at depolarized membrane potentials. Cytosolic Ca2+ oscillations observed at holding potentials of -20 to -80 mV were terminated by holding at -10 mV or more depolarized potentials. The frequency of Ca2+ oscillations increased with membrane hyperpolarization. Bradykinin significantly enhanced the hyperpolarization-induced increases in the intracellular free Ca2+ concentration ([Ca2+]i) upon membrane hyperpolarization only in DT cells but not in control cells. No [Ca2+]i increase upon hyperpolarization was observed in bradykinin-stimulated DT cells in the absence of external Ca2+, suggesting that bradykinin activates Ca2+ influx. [Ca2+]i increased upon application of inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4) into control and DT cells in an extracellular Ca(2+)-dependent manner, indicating that NIH/3T3 fibroblasts have an Ins(1,3,4,5)P4-gated Ca2+ influx pathway. Ins-(1,3,4,5)P4, however, produced the sustained [Ca2+]i increase in DT cells, but not in control NIH/3T3 cells, suggesting that ras may lock the Ca2+ influx pathway at the activated state. Cytosolic Ca2+ oscillations, bradykinin-enhanced Ca2+ influx, and Ins(1,3,4,5)P4-induced Ca2+ influx were all similar in that activity was increased by membrane hyperpolarization. The results suggest that bradykinin-induced cytosolic Ca2+ oscillations in ras-transformed NIH/3T3 cells are maintained by bradykinin-activated continuous Ca2+ influx which may use Ins(1,3,4,5)P4 as an intracellular messenger." @default.
• W1604367031 created "2016-06-24" @default.
• W1604367031 creator A5049786044 @default.
• W1604367031 creator A5077107266 @default.
• W1604367031 creator A5087695533 @default.
• W1604367031 date "1993-09-01" @default.
• W1604367031 modified "2023-09-29" @default.
• W1604367031 title "Bradykinin-induced cytosolic Ca2+ oscillations and inositol tetrakisphosphate-induced Ca2+ influx in voltage-clamped ras-transformed NIH/3T3 fibroblasts." @default.
• W1604367031 cites W1482458371 @default.
• W1604367031 cites W1490783082 @default.
• W1604367031 cites W1502161210 @default.
• W1604367031 cites W1518984641 @default.
• W1604367031 cites W1531851959 @default.
• W1604367031 cites W1533466089 @default.
• W1604367031 cites W1535912510 @default.
• W1604367031 cites W1546078475 @default.
• W1604367031 cites W1566907047 @default.
• W1604367031 cites W1576416454 @default.
• W1604367031 cites W1591067042 @default.
• W1604367031 cites W1596708629 @default.
• W1604367031 cites W1605633986 @default.
• W1604367031 cites W1669858368 @default.
• W1604367031 cites W1833275968 @default.
• W1604367031 cites W1886794638 @default.
• W1604367031 cites W1964559136 @default.
• W1604367031 cites W1966427545 @default.
• W1604367031 cites W1968190346 @default.
• W1604367031 cites W1969006090 @default.
• W1604367031 cites W1971754412 @default.
• W1604367031 cites W1973237558 @default.
• W1604367031 cites W1978621039 @default.
• W1604367031 cites W1979102998 @default.
• W1604367031 cites W1980166477 @default.
• W1604367031 cites W1986285883 @default.
• W1604367031 cites W1990074560 @default.
• W1604367031 cites W1997093700 @default.
• W1604367031 cites W1999190644 @default.
• W1604367031 cites W2001417329 @default.
• W1604367031 cites W2002845695 @default.
• W1604367031 cites W2004589688 @default.
• W1604367031 cites W2009618739 @default.
• W1604367031 cites W2009667219 @default.
• W1604367031 cites W2009935511 @default.
• W1604367031 cites W2014543453 @default.
• W1604367031 cites W2014988945 @default.
• W1604367031 cites W2015480034 @default.
• W1604367031 cites W2016219644 @default.
• W1604367031 cites W2024771423 @default.
• W1604367031 cites W2026478305 @default.
• W1604367031 cites W2032144030 @default.
• W1604367031 cites W2035034385 @default.
• W1604367031 cites W2038531813 @default.
• W1604367031 cites W2045673068 @default.
• W1604367031 cites W2047101291 @default.
• W1604367031 cites W2054320393 @default.
• W1604367031 cites W2061645562 @default.
• W1604367031 cites W2062801239 @default.
• W1604367031 cites W2071857701 @default.
• W1604367031 cites W2078610883 @default.
• W1604367031 cites W2086449273 @default.
• W1604367031 cites W2091619553 @default.
• W1604367031 cites W2114401576 @default.
• W1604367031 cites W2126559707 @default.
• W1604367031 cites W2141260882 @default.
• W1604367031 cites W2141679273 @default.
• W1604367031 cites W2174190166 @default.
• W1604367031 cites W2204513816 @default.
• W1604367031 cites W2336755139 @default.
• W1604367031 cites W2408825431 @default.
• W1604367031 cites W2460311538 @default.
• W1604367031 cites W2799503982 @default.
• W1604367031 cites W4238781098 @default.
• W1604367031 doi "https://doi.org/10.1016/s0021-9258(19)36529-9" @default.
• W1604367031 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8366088" @default.
• W1604367031 hasPublicationYear "1993" @default.
• W1604367031 type Work @default.
• W1604367031 sameAs 1604367031 @default.
• W1604367031 citedByCount "43" @default.
• W1604367031 countsByYear W16043670312012 @default.
• W1604367031 countsByYear W16043670312014 @default.
• W1604367031 countsByYear W16043670312016 @default.
• W1604367031 countsByYear W16043670312018 @default.
• W1604367031 crossrefType "journal-article" @default.
• W1604367031 hasAuthorship W1604367031A5049786044 @default.
• W1604367031 hasAuthorship W1604367031A5077107266 @default.
• W1604367031 hasAuthorship W1604367031A5087695533 @default.
• W1604367031 hasBestOaLocation W16043670311 @default.
• W1604367031 hasConcept C104317684 @default.
• W1604367031 hasConcept C12554922 @default.
• W1604367031 hasConcept C126322002 @default.
• W1604367031 hasConcept C134018914 @default.
• W1604367031 hasConcept C170493617 @default.
• W1604367031 hasConcept C181199279 @default.
• W1604367031 hasConcept C185592680 @default.
• W1604367031 hasConcept C2776746612 @default.
• W1604367031 hasConcept C2777427919 @default.
• W1604367031 hasConcept C2779591629 @default.
• W1604367031 hasConcept C54009773 @default.

• next