FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1604699580> ?p ?o ?g. }

• W1604699580 endingPage "2116" @default.
• W1604699580 startingPage "2107" @default.
• W1604699580 abstract "The Baeyer–Villiger monooxygenase (BVMO), 4-hydroxyacetophenone monooxygenase (HAPMO), uses NADPH and O2 to oxidize a variety of aromatic ketones and sulfides. The FAD-containing enzyme has a 700-fold preference for NADPH over NADH. Sequence alignment with other BVMOs, which are all known to be selective for NADPH, revealed three conserved basic residues, which could account for the observed coenzyme specificity. The corresponding residues in HAPMO (Arg339, Lys439 and Arg440) were mutated and the properties of the purified mutant enzymes were studied. For Arg440 no involvement in coenzyme recognition could be shown as mutant R440A was totally inactive. Although this mutant could still be fully reduced by NADPH, no oxygenation occurred, indicating that this residue is crucial for completing the catalytic cycle of HAPMO. Characterization of several Arg339 and Lys439 mutants revealed that these residues are indeed both involved in coenzyme recognition. Mutant R339A showed a largely decreased affinity for NADPH, as judged from kinetic analysis and binding experiments. Replacing Arg339 also resulted in a decreased catalytic efficiency with NADH. Mutant K439A displayed a 100-fold decrease in catalytic efficiency with NADPH, mainly caused by an increased Km. However, the efficiency with NADH increased fourfold. Saturation mutagenesis at position 439 showed that the presence of an asparagine or a phenylalanine improves the catalytic efficiency with NADH by a factor of 6 to 7. All Lys439 mutants displayed a lower affinity for AADP+, confirming a role of the lysine in recognizing the 2′-phosphate of NADPH. The results obtained could be extrapolated to the sequence-related cyclohexanone monooxygenase. Replacing Lys326 in this BVMO, which is analogous to Lys439 in HAPMO, again changed the coenzyme specificity towards NADH. These results indicate that the strict NADPH dependency of this class of monooxygenases is based upon recognition of the coenzyme by several basic residues." @default.
• W1604699580 created "2016-06-24" @default.
• W1604699580 creator A5023328642 @default.
• W1604699580 creator A5076930474 @default.
• W1604699580 creator A5083203989 @default.
• W1604699580 date "2004-05-10" @default.
• W1604699580 modified "2023-10-16" @default.
• W1604699580 title "Identifying determinants of NADPH specificity in Baeyer-Villiger monooxygenases" @default.
• W1604699580 cites W1489973189 @default.
• W1604699580 cites W1556162770 @default.
• W1604699580 cites W1590566609 @default.
• W1604699580 cites W1599238750 @default.
• W1604699580 cites W1977552539 @default.
• W1604699580 cites W1989365471 @default.
• W1604699580 cites W1990516510 @default.
• W1604699580 cites W1990795007 @default.
• W1604699580 cites W1991908994 @default.
• W1604699580 cites W1997844120 @default.
• W1604699580 cites W1999842012 @default.
• W1604699580 cites W2001709760 @default.
• W1604699580 cites W2008000048 @default.
• W1604699580 cites W2024327378 @default.
• W1604699580 cites W2025006941 @default.
• W1604699580 cites W2026452542 @default.
• W1604699580 cites W2027294022 @default.
• W1604699580 cites W2037580586 @default.
• W1604699580 cites W2038059557 @default.
• W1604699580 cites W2043421474 @default.
• W1604699580 cites W2043510269 @default.
• W1604699580 cites W2045983474 @default.
• W1604699580 cites W2054492447 @default.
• W1604699580 cites W2056418197 @default.
• W1604699580 cites W2057317771 @default.
• W1604699580 cites W2059890422 @default.
• W1604699580 cites W2061806933 @default.
• W1604699580 cites W2064872811 @default.
• W1604699580 cites W2075380931 @default.
• W1604699580 cites W2083470544 @default.
• W1604699580 cites W2085584165 @default.
• W1604699580 cites W2091957029 @default.
• W1604699580 cites W2093826857 @default.
• W1604699580 cites W2100498403 @default.
• W1604699580 cites W2106419599 @default.
• W1604699580 cites W2110850548 @default.
• W1604699580 cites W2114878375 @default.
• W1604699580 cites W2116960243 @default.
• W1604699580 cites W2120113652 @default.
• W1604699580 cites W2128541897 @default.
• W1604699580 cites W2128804683 @default.
• W1604699580 cites W2130913111 @default.
• W1604699580 cites W2135501966 @default.
• W1604699580 cites W2138535824 @default.
• W1604699580 cites W2149617706 @default.
• W1604699580 cites W2153047963 @default.
• W1604699580 cites W2154934782 @default.
• W1604699580 cites W2155024334 @default.
• W1604699580 cites W2160143801 @default.
• W1604699580 cites W4210990914 @default.
• W1604699580 cites W4293247451 @default.
• W1604699580 doi "https://doi.org/10.1111/j.1432-1033.2004.04126.x" @default.
• W1604699580 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15153101" @default.
• W1604699580 hasPublicationYear "2004" @default.
• W1604699580 type Work @default.
• W1604699580 sameAs 1604699580 @default.
• W1604699580 citedByCount "65" @default.
• W1604699580 countsByYear W16046995802012 @default.
• W1604699580 countsByYear W16046995802013 @default.
• W1604699580 countsByYear W16046995802014 @default.
• W1604699580 countsByYear W16046995802016 @default.
• W1604699580 countsByYear W16046995802017 @default.
• W1604699580 countsByYear W16046995802018 @default.
• W1604699580 countsByYear W16046995802019 @default.
• W1604699580 countsByYear W16046995802020 @default.
• W1604699580 countsByYear W16046995802021 @default.
• W1604699580 countsByYear W16046995802022 @default.
• W1604699580 countsByYear W16046995802023 @default.
• W1604699580 crossrefType "journal-article" @default.
• W1604699580 hasAuthorship W1604699580A5023328642 @default.
• W1604699580 hasAuthorship W1604699580A5076930474 @default.
• W1604699580 hasAuthorship W1604699580A5083203989 @default.
• W1604699580 hasBestOaLocation W16046995801 @default.
• W1604699580 hasConcept C103408237 @default.
• W1604699580 hasConcept C104317684 @default.
• W1604699580 hasConcept C109129352 @default.
• W1604699580 hasConcept C13002179 @default.
• W1604699580 hasConcept C143065580 @default.
• W1604699580 hasConcept C16318435 @default.
• W1604699580 hasConcept C181199279 @default.
• W1604699580 hasConcept C185592680 @default.
• W1604699580 hasConcept C197957613 @default.
• W1604699580 hasConcept C2775895851 @default.
• W1604699580 hasConcept C2781338088 @default.
• W1604699580 hasConcept C526171541 @default.
• W1604699580 hasConcept C55493867 @default.
• W1604699580 hasConcept C71240020 @default.
• W1604699580 hasConcept C86803240 @default.
• W1604699580 hasConceptScore W1604699580C103408237 @default.
• W1604699580 hasConceptScore W1604699580C104317684 @default.

• next