FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1604730280> ?p ?o ?g. }

• W1604730280 endingPage "358" @default.
• W1604730280 startingPage "353" @default.
• W1604730280 abstract "Abstract Noninvasive biomarkers hold important potential for the characterization and purification of stem cells because the addition of exogenous labels, probes, or reporters, as well as the disruption of cell‐cell and cell‐extracellular matrix interactions, can unintentionally but dramatically alter stem cell state. We recently showed that intensity of the intrinsically fluorescent metabolite, nicotinamide adenine dinucleotide (NADH), fluctuates predictably with changes in stem cell viability and differentiation state. Here, we use multiphoton flow cytometry developed in our laboratory to rapidly and noninvasively characterize and purify populations of intact stem cell aggregates based on NADH intensity and assessed the differentiation capacity of sorted populations. We found removal of aggregates with NADH intensity indicative of cell death resulted in a remaining population of aggregates significantly more likely to produce beating cardiomyocytes (26% vs. 8%, P < 0.05). Similarly, we found isolation of stem cell aggregates with NADH intensity indicative of future cardiac differentiation gave rise to more aggregates with beating cardiomyocytes at later time points (50% vs. 28%, P < 0.05). Further, coupling NADH intensity with gating based on size, enhances the enrichment for EBs capable of giving rise to cardiomyocytes (59% vs. 27%, P < 0.05). Thus, we demonstrate that endogenous properties of cell aggregates, such as NADH and size, can serve as gating parameters for large particle sorting devices to purify populations of stem cells or their progeny in a noninvasive manner, leading the way for improved therapeutic applications. © 2014 International Society for Advancement of Cytometry" @default.
• W1604730280 created "2016-06-24" @default.
• W1604730280 creator A5010413374 @default.
• W1604730280 creator A5035080304 @default.
• W1604730280 creator A5058517738 @default.
• W1604730280 creator A5072668321 @default.
• W1604730280 creator A5078540488 @default.
• W1604730280 creator A5082090157 @default.
• W1604730280 date "2014-01-17" @default.
• W1604730280 modified "2023-10-16" @default.
• W1604730280 title "Noninvasive sorting of stem cell aggregates based on intrinsic markers" @default.
• W1604730280 cites W1979968811 @default.
• W1604730280 cites W1981789666 @default.
• W1604730280 cites W1983962289 @default.
• W1604730280 cites W1993048177 @default.
• W1604730280 cites W1993671248 @default.
• W1604730280 cites W1997910229 @default.
• W1604730280 cites W2002828554 @default.
• W1604730280 cites W2008289317 @default.
• W1604730280 cites W2008932213 @default.
• W1604730280 cites W2013412105 @default.
• W1604730280 cites W2015049254 @default.
• W1604730280 cites W2019240387 @default.
• W1604730280 cites W2034356218 @default.
• W1604730280 cites W2035341604 @default.
• W1604730280 cites W2040331321 @default.
• W1604730280 cites W2041850918 @default.
• W1604730280 cites W2048722591 @default.
• W1604730280 cites W2051029261 @default.
• W1604730280 cites W2061464631 @default.
• W1604730280 cites W2062944461 @default.
• W1604730280 cites W2064124462 @default.
• W1604730280 cites W2068078989 @default.
• W1604730280 cites W2071457705 @default.
• W1604730280 cites W2072227605 @default.
• W1604730280 cites W2081972846 @default.
• W1604730280 cites W2093923168 @default.
• W1604730280 cites W2111812837 @default.
• W1604730280 cites W2111920179 @default.
• W1604730280 cites W2117462238 @default.
• W1604730280 cites W2123607881 @default.
• W1604730280 cites W2131277527 @default.
• W1604730280 cites W2142008191 @default.
• W1604730280 cites W2142449420 @default.
• W1604730280 cites W2143852617 @default.
• W1604730280 cites W2148414537 @default.
• W1604730280 cites W2159266673 @default.
• W1604730280 cites W2170941561 @default.
• W1604730280 cites W2333274863 @default.
• W1604730280 cites W2462814986 @default.
• W1604730280 doi "https://doi.org/10.1002/cyto.a.22436" @default.
• W1604730280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24443408" @default.
• W1604730280 hasPublicationYear "2014" @default.
• W1604730280 type Work @default.
• W1604730280 sameAs 1604730280 @default.
• W1604730280 citedByCount "4" @default.
• W1604730280 countsByYear W16047302802015 @default.
• W1604730280 countsByYear W16047302802018 @default.
• W1604730280 countsByYear W16047302802019 @default.
• W1604730280 crossrefType "journal-article" @default.
• W1604730280 hasAuthorship W1604730280A5010413374 @default.
• W1604730280 hasAuthorship W1604730280A5035080304 @default.
• W1604730280 hasAuthorship W1604730280A5058517738 @default.
• W1604730280 hasAuthorship W1604730280A5072668321 @default.
• W1604730280 hasAuthorship W1604730280A5078540488 @default.
• W1604730280 hasAuthorship W1604730280A5082090157 @default.
• W1604730280 hasBestOaLocation W16047302801 @default.
• W1604730280 hasConcept C12554922 @default.
• W1604730280 hasConcept C1491633281 @default.
• W1604730280 hasConcept C153911025 @default.
• W1604730280 hasConcept C181199279 @default.
• W1604730280 hasConcept C185592680 @default.
• W1604730280 hasConcept C2776901170 @default.
• W1604730280 hasConcept C2778630268 @default.
• W1604730280 hasConcept C28328180 @default.
• W1604730280 hasConcept C28406088 @default.
• W1604730280 hasConcept C2908647359 @default.
• W1604730280 hasConcept C53227056 @default.
• W1604730280 hasConcept C553184892 @default.
• W1604730280 hasConcept C55493867 @default.
• W1604730280 hasConcept C71924100 @default.
• W1604730280 hasConcept C75520062 @default.
• W1604730280 hasConcept C79879829 @default.
• W1604730280 hasConcept C86803240 @default.
• W1604730280 hasConcept C95444343 @default.
• W1604730280 hasConcept C99454951 @default.
• W1604730280 hasConceptScore W1604730280C12554922 @default.
• W1604730280 hasConceptScore W1604730280C1491633281 @default.
• W1604730280 hasConceptScore W1604730280C153911025 @default.
• W1604730280 hasConceptScore W1604730280C181199279 @default.
• W1604730280 hasConceptScore W1604730280C185592680 @default.
• W1604730280 hasConceptScore W1604730280C2776901170 @default.
• W1604730280 hasConceptScore W1604730280C2778630268 @default.
• W1604730280 hasConceptScore W1604730280C28328180 @default.
• W1604730280 hasConceptScore W1604730280C28406088 @default.
• W1604730280 hasConceptScore W1604730280C2908647359 @default.
• W1604730280 hasConceptScore W1604730280C53227056 @default.
• W1604730280 hasConceptScore W1604730280C553184892 @default.

• next