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- W1605194916 abstract "Abstract Isolated perfused rat livers were dispersed by a two-step procedure of Ca2+ removal (probably including the removal of a Ca2+-dependent adhesion factor) followed by enzymatic treatment. Collagenase, crude or purified, converted all of the parenchymal tissue to a suspension of cells which were 95% intact. Other enzymes such as hyaluronidase, lysozyme and trypsin were ineffective. Since hypoxic conditions during enzymatic treatment did not affect cellular viability, the oxygenation apparatus can be omitted to facilitate sterile preparation of cells. Purification of the parenchymal cells by differential centrifugation reduced contamination by non-parenchymal cells from 10–20% initially to 1–2% finally, with 40% of the parenchymal cells recovered and no loss in viability. The purified cells were 20% binucleated and had the same biochemical composition as intact liver tissue. The cells were active in the synthesis of RNA, protein, glycogen and various metabolites, and showed sensitivity towards steroid and polypeptide hormones. Many of the freshly isolated cells had marked constriction furrows which disappeared upon incubation." @default.
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- W1605194916 date "1973-12-01" @default.
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- W1605194916 title "Preparation of rat liver cells" @default.
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- W1605194916 doi "https://doi.org/10.1016/0014-4827(73)90357-1" @default.
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