FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1605635614> ?p ?o ?g. }

• W1605635614 endingPage "11956" @default.
• W1605635614 startingPage "11949" @default.
• W1605635614 abstract "Human fibroblasts secrete insulin-like growth factor-binding proteins (IGFBPs) that can modify insulin-like growth factor (IGF) I action. We have determined the molecular identities of three forms of IGFBPs that are secreted by human fibroblasts in vitro. Ligand blot analysis of fibroblast conditioned media revealed that the M(r) 43,000 and 39,000 forms were the most abundant, but that M(r) 31,000 and 24,000 forms were also present. An antiserum that was specific for IGFBP-5 reacted with the M(r) 31,000 form, and an IGFBP-4-specific antiserum recognized only the M(r) 24,000 form. The M(r) 39,000 and 43,000 forms were detected by IGFBP-3 antiserum. Further proof that fibroblasts synthesized these forms of IGFBPs was obtained by Northern blotting. A cDNA probe for IGFBP-3 hybridized with a 2.4-kilobase (kb) transcript, whereas a cDNA probe for IGFBP-5 recognized a single 6.0-kb transcript, and an IGFBP-4 cDNA probe recognized 2.2- and 2.0-kb transcripts. IGF-I and -II caused a minimal (less than 43%) increase in IGFBP-5 mRNA abundance and had no effect on IGFBP-4 mRNA abundance. IGF-I and -II (100 ng/ml) stimulated 6-8-fold increases in IGFBP-5 levels, whereas IGFBP-4 was inhibited. Insulin failed to elicit any change in IGFBP-5, suggesting that binding of the IGFs to IGFBPs was required to detect the increase. Immunoblotting for IGFBP-5 revealed an M(r) 23,000 (non-IGF-I-binding) fragment. To determine if the IGFs were influencing proteolytic degradation of IGFBP-5, pure IGFBP-5 was added to fibroblast cultures and incubated for 4 h at 37 degrees C. The amount of fragment formation was attenuated by the presence of IGF-I and -II, but not insulin, suggesting that this is a mechanism by which the IGFs act to modulate IGFBP-5 concentration. In contrast to the IGFs, forskolin, which increased IGFBP-4 and -5 mRNA abundance and secretion, had no effect on fragment formation. The results show that human fibroblasts synthesize and secrete IGFBP-3, -4, and -5 and that changes in intracellular cAMP regulate synthesis, whereas the IGFs regulate IGFBP-4 and -5 levels by post-transcriptional mechanisms." @default.
• W1605635614 created "2016-06-24" @default.
• W1605635614 creator A5000288481 @default.
• W1605635614 creator A5005642782 @default.
• W1605635614 creator A5039368198 @default.
• W1605635614 creator A5047854064 @default.
• W1605635614 creator A5060793471 @default.
• W1605635614 date "1992-06-01" @default.
• W1605635614 modified "2023-10-15" @default.
• W1605635614 title "Identification of the forms of insulin-like growth factor-binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion." @default.
• W1605635614 cites W1489237025 @default.
• W1605635614 cites W1512320531 @default.
• W1605635614 cites W1547915919 @default.
• W1605635614 cites W1571404318 @default.
• W1605635614 cites W1972819401 @default.
• W1605635614 cites W1975949772 @default.
• W1605635614 cites W1992668278 @default.
• W1605635614 cites W2002841462 @default.
• W1605635614 cites W2003016121 @default.
• W1605635614 cites W2006156624 @default.
• W1605635614 cites W2017309664 @default.
• W1605635614 cites W2018723236 @default.
• W1605635614 cites W2027077889 @default.
• W1605635614 cites W2032558447 @default.
• W1605635614 cites W2032776824 @default.
• W1605635614 cites W2053199384 @default.
• W1605635614 cites W2058870799 @default.
• W1605635614 cites W2059037233 @default.
• W1605635614 cites W2064719012 @default.
• W1605635614 cites W2072159062 @default.
• W1605635614 cites W2083406280 @default.
• W1605635614 cites W2083891157 @default.
• W1605635614 cites W2085704247 @default.
• W1605635614 cites W2094466007 @default.
• W1605635614 cites W2100837269 @default.
• W1605635614 cites W2104530948 @default.
• W1605635614 cites W2138270253 @default.
• W1605635614 cites W2140204001 @default.
• W1605635614 cites W2151261265 @default.
• W1605635614 cites W2169995876 @default.
• W1605635614 cites W2224929038 @default.
• W1605635614 cites W2576734941 @default.
• W1605635614 cites W4294216491 @default.
• W1605635614 doi "https://doi.org/10.1016/s0021-9258(19)49788-3" @default.
• W1605635614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1376315" @default.
• W1605635614 hasPublicationYear "1992" @default.
• W1605635614 type Work @default.
• W1605635614 sameAs 1605635614 @default.
• W1605635614 citedByCount "262" @default.
• W1605635614 countsByYear W16056356142012 @default.
• W1605635614 countsByYear W16056356142013 @default.
• W1605635614 countsByYear W16056356142014 @default.
• W1605635614 countsByYear W16056356142016 @default.
• W1605635614 countsByYear W16056356142017 @default.
• W1605635614 countsByYear W16056356142018 @default.
• W1605635614 countsByYear W16056356142020 @default.
• W1605635614 countsByYear W16056356142021 @default.
• W1605635614 crossrefType "journal-article" @default.
• W1605635614 hasAuthorship W1605635614A5000288481 @default.
• W1605635614 hasAuthorship W1605635614A5005642782 @default.
• W1605635614 hasAuthorship W1605635614A5039368198 @default.
• W1605635614 hasAuthorship W1605635614A5047854064 @default.
• W1605635614 hasAuthorship W1605635614A5060793471 @default.
• W1605635614 hasBestOaLocation W16056356141 @default.
• W1605635614 hasConcept C116834253 @default.
• W1605635614 hasConcept C134018914 @default.
• W1605635614 hasConcept C170493617 @default.
• W1605635614 hasConcept C185592680 @default.
• W1605635614 hasConcept C2775960820 @default.
• W1605635614 hasConcept C2779306644 @default.
• W1605635614 hasConcept C2780378035 @default.
• W1605635614 hasConcept C2780689927 @default.
• W1605635614 hasConcept C49039625 @default.
• W1605635614 hasConcept C51639874 @default.
• W1605635614 hasConcept C55493867 @default.
• W1605635614 hasConcept C59822182 @default.
• W1605635614 hasConcept C86803240 @default.
• W1605635614 hasConcept C95444343 @default.
• W1605635614 hasConceptScore W1605635614C116834253 @default.
• W1605635614 hasConceptScore W1605635614C134018914 @default.
• W1605635614 hasConceptScore W1605635614C170493617 @default.
• W1605635614 hasConceptScore W1605635614C185592680 @default.
• W1605635614 hasConceptScore W1605635614C2775960820 @default.
• W1605635614 hasConceptScore W1605635614C2779306644 @default.
• W1605635614 hasConceptScore W1605635614C2780378035 @default.
• W1605635614 hasConceptScore W1605635614C2780689927 @default.
• W1605635614 hasConceptScore W1605635614C49039625 @default.
• W1605635614 hasConceptScore W1605635614C51639874 @default.
• W1605635614 hasConceptScore W1605635614C55493867 @default.
• W1605635614 hasConceptScore W1605635614C59822182 @default.
• W1605635614 hasConceptScore W1605635614C86803240 @default.
• W1605635614 hasConceptScore W1605635614C95444343 @default.
• W1605635614 hasIssue "17" @default.
• W1605635614 hasLocation W16056356141 @default.
• W1605635614 hasLocation W16056356142 @default.
• W1605635614 hasOpenAccess W1605635614 @default.
• W1605635614 hasPrimaryLocation W16056356141 @default.
• W1605635614 hasRelatedWork W1994750899 @default.

• next