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- W1605767025 abstract "A confirmatory high‐pressure liquid chromatographic method for the determination of nine tetracyclines in S parus aurata (gilthead seabream) muscle tissue is developed and presented herein. Tetracycline, 4‐epi‐tetracycline, oxytetracycline, 4‐epi‐oxytetracycline, chlortetracycline, 4‐epi‐chlortetracycline, doxycycline, methacycline and demeclocycline were separated on a K romasil, C 18 (250 mm × 4 mm, 5 μm) analytical column by gradient elution with a mobile phase consisting of 0.001 M ethylenediaminetetraacetic acid/sodium salt and acetonitrile at 25°C. Diode array detection with monitoring at 280 nm (for the determination of chlortetracycline, 4‐epi‐chlortetracycline, methacycline and demeclocycline) and 355 nm (for tetracycline, 4‐epi‐tetracycline, oxytetracycline, 4‐epi‐oxytetracycline and demeclocycline) was applied for peak identification and quantification of analytes. Examined antibiotics were isolated from gilthead seabream tissue by leaching using a citrate buffer (pH 4.0) and purified by solid phase extraction using O asis HLB (200 mg/6 mL) cartridges. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the E uropean U nion D ecision 2002/657/ EC ." @default.
- W1605767025 created "2016-06-24" @default.
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- W1605767025 date "2012-06-01" @default.
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- W1605767025 title "Confirmatory development and validation of HPLC-DADmethod for the determination of tetracyclines in gilthead seabream (Sparus aurata) muscle tissue" @default.
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- W1605767025 doi "https://doi.org/10.1002/jssc.201100996" @default.
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