FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1605897467> ?p ?o ?g. }

• W1605897467 endingPage "993" @default.
• W1605897467 startingPage "988" @default.
• W1605897467 abstract "The increasing uses of DNA methodologies to study the micro flora of the pig gastrointestinal tract requires an efficient recovery of bacterial DNA from the intestinal sample. Thus, the objective of this study was to determine which DNA extraction methods are most effective for luminal samples from pigs. Several routinely used nucleic acid extraction procedures were compared based upon quantity and purity of extracted DNA.DNA was extracted from pig colonic and caecal lumen samples using 19 methods for bacterial DNA extraction. The quantity of total DNA recovered by each extraction method was determined and compared. Two methods using extraction with polyvinylpolypyrrolidone (PVPP) or phenol and two methods involving bead mill homogenization were found to provide the greatest quantity of extracted DNA for both colonic and caecal lumen. Extracted DNA from these four methods was further analysed for purity based upon the presence of PCR inhibitors, which was ascertained by determining the efficiency of amplification of a segment of the 16S rDNA. PCR amplification could be readily achieved with DNA extracted by each of these four methods, but efficiency of amplification tended to be higher with DNA from two of the methods (one extracted with PVPP and one with bead mill homogenization).Four extraction methods proved to be significantly superior in quantity of DNA extracted from luminal samples. Of these four, no strong inhibitors of PCR amplification were detected in any of the extracted DNA. However, the efficiency of amplification tended to be lower in DNA samples from two of the methods, suggesting the presence of low levels of PCR inhibitors.Results of this study provide a basis for choosing which DNA extraction procedures are most effective for use with samples of pig lumen." @default.
• W1605897467 created "2016-06-24" @default.
• W1605897467 creator A5009068724 @default.
• W1605897467 creator A5057660867 @default.
• W1605897467 date "2003-05-13" @default.
• W1605897467 modified "2023-10-10" @default.
• W1605897467 title "Comparison of rapid methods for the extraction of bacterial DNA from colonic and caecal lumen contents of the pig" @default.
• W1605897467 cites W1488352592 @default.
• W1605897467 cites W1498206429 @default.
• W1605897467 cites W1514657191 @default.
• W1605897467 cites W1571452544 @default.
• W1605897467 cites W1577668470 @default.
• W1605897467 cites W1955295475 @default.
• W1605897467 cites W1967850793 @default.
• W1605897467 cites W1984637730 @default.
• W1605897467 cites W2003375770 @default.
• W1605897467 cites W2013620980 @default.
• W1605897467 cites W2025877431 @default.
• W1605897467 cites W2032028602 @default.
• W1605897467 cites W2049333188 @default.
• W1605897467 cites W2062232689 @default.
• W1605897467 cites W2084395757 @default.
• W1605897467 cites W2094751929 @default.
• W1605897467 cites W2097979496 @default.
• W1605897467 cites W2101627367 @default.
• W1605897467 cites W2112078166 @default.
• W1605897467 cites W2113799232 @default.
• W1605897467 cites W2122667648 @default.
• W1605897467 cites W2123088611 @default.
• W1605897467 cites W2139672978 @default.
• W1605897467 cites W2153823136 @default.
• W1605897467 cites W2156475459 @default.
• W1605897467 cites W2157783441 @default.
• W1605897467 doi "https://doi.org/10.1046/j.1365-2672.2003.01917.x" @default.
• W1605897467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12752806" @default.
• W1605897467 hasPublicationYear "2003" @default.
• W1605897467 type Work @default.
• W1605897467 sameAs 1605897467 @default.
• W1605897467 citedByCount "24" @default.
• W1605897467 countsByYear W16058974672012 @default.
• W1605897467 countsByYear W16058974672013 @default.
• W1605897467 countsByYear W16058974672014 @default.
• W1605897467 countsByYear W16058974672015 @default.
• W1605897467 countsByYear W16058974672016 @default.
• W1605897467 countsByYear W16058974672019 @default.
• W1605897467 countsByYear W16058974672020 @default.
• W1605897467 countsByYear W16058974672021 @default.
• W1605897467 crossrefType "journal-article" @default.
• W1605897467 hasAuthorship W1605897467A5009068724 @default.
• W1605897467 hasAuthorship W1605897467A5057660867 @default.
• W1605897467 hasConcept C104317684 @default.
• W1605897467 hasConcept C130217890 @default.
• W1605897467 hasConcept C153911025 @default.
• W1605897467 hasConcept C185592680 @default.
• W1605897467 hasConcept C18903297 @default.
• W1605897467 hasConcept C24107716 @default.
• W1605897467 hasConcept C2777507495 @default.
• W1605897467 hasConcept C2778722038 @default.
• W1605897467 hasConcept C43617362 @default.
• W1605897467 hasConcept C4725764 @default.
• W1605897467 hasConcept C49105822 @default.
• W1605897467 hasConcept C552990157 @default.
• W1605897467 hasConcept C55493867 @default.
• W1605897467 hasConcept C78063203 @default.
• W1605897467 hasConcept C86803240 @default.
• W1605897467 hasConcept C89423630 @default.
• W1605897467 hasConceptScore W1605897467C104317684 @default.
• W1605897467 hasConceptScore W1605897467C130217890 @default.
• W1605897467 hasConceptScore W1605897467C153911025 @default.
• W1605897467 hasConceptScore W1605897467C185592680 @default.
• W1605897467 hasConceptScore W1605897467C18903297 @default.
• W1605897467 hasConceptScore W1605897467C24107716 @default.
• W1605897467 hasConceptScore W1605897467C2777507495 @default.
• W1605897467 hasConceptScore W1605897467C2778722038 @default.
• W1605897467 hasConceptScore W1605897467C43617362 @default.
• W1605897467 hasConceptScore W1605897467C4725764 @default.
• W1605897467 hasConceptScore W1605897467C49105822 @default.
• W1605897467 hasConceptScore W1605897467C552990157 @default.
• W1605897467 hasConceptScore W1605897467C55493867 @default.
• W1605897467 hasConceptScore W1605897467C78063203 @default.
• W1605897467 hasConceptScore W1605897467C86803240 @default.
• W1605897467 hasConceptScore W1605897467C89423630 @default.
• W1605897467 hasIssue "6" @default.
• W1605897467 hasLocation W16058974671 @default.
• W1605897467 hasLocation W16058974672 @default.
• W1605897467 hasOpenAccess W1605897467 @default.
• W1605897467 hasPrimaryLocation W16058974671 @default.
• W1605897467 hasRelatedWork W1551630006 @default.
• W1605897467 hasRelatedWork W2085444590 @default.
• W1605897467 hasRelatedWork W2104620528 @default.
• W1605897467 hasRelatedWork W2321924045 @default.
• W1605897467 hasRelatedWork W2330738998 @default.
• W1605897467 hasRelatedWork W2338383269 @default.
• W1605897467 hasRelatedWork W2364423791 @default.
• W1605897467 hasRelatedWork W2460838631 @default.
• W1605897467 hasRelatedWork W3032664268 @default.
• W1605897467 hasRelatedWork W54019796 @default.
• W1605897467 hasVolume "94" @default.

• next