Matches in SemOpenAlex for { <https://semopenalex.org/work/W1605901170> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1605901170 endingPage "95" @default.
- W1605901170 startingPage "82" @default.
- W1605901170 abstract "The binding of proflavine to 30-S, 50-S and 70-S ribosomes has been studied quantitatively utilising spectral changes in the dye to measure the degree of binding. All three particles produced hypochromism in the ultraviolet difference spectrum of the dye and a displacement in the visible spectrum to longer wavelengths with the formation of characteristic isobestic points. An analysis of the spectral changes revealed two distinct binding processes. A strong binding characterised by a free energy of association of 10 kcal/mole appeared to involve Van der Waals interactions between the dye and the bases on the RNA; a weak interaction was attributed to electrostatic binding of the dye to the RNA phosphates. The number of strong binding sites was 96, 70 and 108 for the 30-S, 50-S and 70-S ribosomes, respectively. Despite an increase of about 50 in the number of binding sites when 70-S ribosomes dissociate into their subunits the binding of proflavine to 30-S and 50-S ribosomes did not appear to affect the reassociation of these particles to form 70-S ribosomes. The number of strong binding sites on the 50-S ribosome increased to about 500 when the particle was unfolded in EDTA. This suggested that binding sites, which are exposed in the unfolded particle, are not available for binding dye in the intact ribosome because of the rigid tertiary folding in the native particle." @default.
- W1605901170 created "2016-06-24" @default.
- W1605901170 creator A5017897004 @default.
- W1605901170 creator A5034913303 @default.
- W1605901170 date "1967-08-01" @default.
- W1605901170 modified "2023-09-23" @default.
- W1605901170 title "Structural studies on ribosomes" @default.
- W1605901170 cites W102466847 @default.
- W1605901170 cites W1490179123 @default.
- W1605901170 cites W1515052776 @default.
- W1605901170 cites W1906197854 @default.
- W1605901170 cites W1964079803 @default.
- W1605901170 cites W1983649348 @default.
- W1605901170 cites W1992784057 @default.
- W1605901170 cites W2003061519 @default.
- W1605901170 cites W2012832520 @default.
- W1605901170 cites W2017249533 @default.
- W1605901170 cites W2019446780 @default.
- W1605901170 cites W2041714328 @default.
- W1605901170 cites W2062110118 @default.
- W1605901170 cites W2064431093 @default.
- W1605901170 cites W2067878166 @default.
- W1605901170 cites W2080509124 @default.
- W1605901170 cites W2168644924 @default.
- W1605901170 cites W2337025505 @default.
- W1605901170 doi "https://doi.org/10.1016/0005-2787(67)90657-0" @default.
- W1605901170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4861263" @default.
- W1605901170 hasPublicationYear "1967" @default.
- W1605901170 type Work @default.
- W1605901170 sameAs 1605901170 @default.
- W1605901170 citedByCount "24" @default.
- W1605901170 countsByYear W16059011702013 @default.
- W1605901170 crossrefType "journal-article" @default.
- W1605901170 hasAuthorship W1605901170A5017897004 @default.
- W1605901170 hasAuthorship W1605901170A5034913303 @default.
- W1605901170 hasConcept C104317684 @default.
- W1605901170 hasConcept C107824862 @default.
- W1605901170 hasConcept C119599485 @default.
- W1605901170 hasConcept C121332964 @default.
- W1605901170 hasConcept C12554922 @default.
- W1605901170 hasConcept C126061179 @default.
- W1605901170 hasConcept C127413603 @default.
- W1605901170 hasConcept C178790620 @default.
- W1605901170 hasConcept C185544564 @default.
- W1605901170 hasConcept C185592680 @default.
- W1605901170 hasConcept C204389451 @default.
- W1605901170 hasConcept C2776545253 @default.
- W1605901170 hasConcept C2776594999 @default.
- W1605901170 hasConcept C32909587 @default.
- W1605901170 hasConcept C552990157 @default.
- W1605901170 hasConcept C55493867 @default.
- W1605901170 hasConcept C67705224 @default.
- W1605901170 hasConcept C8010536 @default.
- W1605901170 hasConcept C86803240 @default.
- W1605901170 hasConcept C88478588 @default.
- W1605901170 hasConcept C93282013 @default.
- W1605901170 hasConceptScore W1605901170C104317684 @default.
- W1605901170 hasConceptScore W1605901170C107824862 @default.
- W1605901170 hasConceptScore W1605901170C119599485 @default.
- W1605901170 hasConceptScore W1605901170C121332964 @default.
- W1605901170 hasConceptScore W1605901170C12554922 @default.
- W1605901170 hasConceptScore W1605901170C126061179 @default.
- W1605901170 hasConceptScore W1605901170C127413603 @default.
- W1605901170 hasConceptScore W1605901170C178790620 @default.
- W1605901170 hasConceptScore W1605901170C185544564 @default.
- W1605901170 hasConceptScore W1605901170C185592680 @default.
- W1605901170 hasConceptScore W1605901170C204389451 @default.
- W1605901170 hasConceptScore W1605901170C2776545253 @default.
- W1605901170 hasConceptScore W1605901170C2776594999 @default.
- W1605901170 hasConceptScore W1605901170C32909587 @default.
- W1605901170 hasConceptScore W1605901170C552990157 @default.
- W1605901170 hasConceptScore W1605901170C55493867 @default.
- W1605901170 hasConceptScore W1605901170C67705224 @default.
- W1605901170 hasConceptScore W1605901170C8010536 @default.
- W1605901170 hasConceptScore W1605901170C86803240 @default.
- W1605901170 hasConceptScore W1605901170C88478588 @default.
- W1605901170 hasConceptScore W1605901170C93282013 @default.
- W1605901170 hasIssue "1" @default.
- W1605901170 hasLocation W16059011701 @default.
- W1605901170 hasLocation W16059011702 @default.
- W1605901170 hasOpenAccess W1605901170 @default.
- W1605901170 hasPrimaryLocation W16059011701 @default.
- W1605901170 hasRelatedWork W117068369 @default.
- W1605901170 hasRelatedWork W1547815808 @default.
- W1605901170 hasRelatedWork W1605901170 @default.
- W1605901170 hasRelatedWork W1968393987 @default.
- W1605901170 hasRelatedWork W1992043237 @default.
- W1605901170 hasRelatedWork W2008935619 @default.
- W1605901170 hasRelatedWork W2262187035 @default.
- W1605901170 hasRelatedWork W2291328383 @default.
- W1605901170 hasRelatedWork W2464535170 @default.
- W1605901170 hasRelatedWork W2913150209 @default.
- W1605901170 hasVolume "145" @default.
- W1605901170 isParatext "false" @default.
- W1605901170 isRetracted "false" @default.
- W1605901170 magId "1605901170" @default.
- W1605901170 workType "article" @default.