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• W1606135044 abstract "Plant regeneration from single or few cells is a prerequisite for effective selection oftransformed cells and to minimize the event of chimeras during transformation. This canonly be achieved if plants are regenerated through callus initiation. To date immatureembryos have been widely used as explants for maize (Zea mays L.) plant regenerationthrough callus initiation and transformation work. However, the utilization of immatureembryos has been hampered by their strictly limited suitable stage for culture, 12-17 daysafter pollination. In contrast mature seeds are ubiquitous. Therefore, use of matureembryos as an explant can significantly reduce the time required to generate immatureembryos and hence the overall time required to regenerate maize plant. However, tropicalmaize genotypes and mature embryos have been considered as the most recalcitrant fortissue culture work. Consequently tropical maize line regeneration using mature embryoshas not been reported so far. The purpose of this study was to regenerate two tropicalmaize lines, CML 216 and Katumani, from mature embryo. Splitting maize seedslongitudinally exposes three different tissues of the embryo simultaneously: scutellum,coleoptile-ring and shoot apical meristem. In the present study up to 92.6% germinationand 0% contamination rate was attained for mature embryos harvested directly from openfield or screen house by soaking sterilized seeds in 1% NaOCI solution for 2-3 hours.Seeds were germinated on MS media supplemented with 2 mg r' 2,4-D. Both the amountand frequency of callus produced by splitting mature seeds early (one day aftergermination) was found to be low, 43.3 % and 57.4 % for Katumani and CML 216genotypes respectively, as compared to 66.3 % and 75.7% for Katumani and CML 216respectively when splitting was done late (3-5 days after germination). The maximumaverage callus induction recorded was 90% for CML 216, 80% for Katumani and 34.3%for A188. When 2,4-D was combined with lower levels of Kinetin ( cytokinin) both theamount and frequency of callus induction was reduced to 52.5% for CML 216 andKatumani and to 34.3% for A188. The media used was LS salts and B5 vitaminssupplemented with 900 mg t', 250 mg r' and 3-4 mg r' of 2,4-D. The averageproduction of Type II and Type I callus was 75.6% and 62.3% respectively. The mediaused was LS salts and B5 vitamins supplemented with 900 mg r', 250 mg r' and 2 mg r'of 2,4-D. The frequency of regenerable calli produced was 21.14% for CML 216 and16.51% for Katumani. The number of shoots regenerated per callus induced from singlesplit seed ranged from 1-5. The media used was LS salts and B5 vitamins supplementedwith 900 mg r', 250 mg r' and 4 mg r' of BAP and 2 mg rl of Kinetin. Plants wereacclimatized in pots contained pit moss. This regeneration protocol gives an alternativeexplant source for maize researchers of the tropics in transgenic maize production totackle different production constraints." @default.
• W1606135044 created "2016-06-24" @default.
• W1606135044 creator A5029896098 @default.
• W1606135044 date "2014-01-11" @default.
• W1606135044 modified "2023-09-26" @default.
• W1606135044 title "Regeneration of two maize genotypes (Zea mays L) from mature embryos through callus initiation using split seed technique" @default.
• W1606135044 hasPublicationYear "2014" @default.
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