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• W1606270170 abstract "Research Article1 April 1988free access Tissue-specific expression of a vimentin–desmin hybrid gene in transgenic mice. P. J. Krimpenfort P. J. Krimpenfort Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author G. Schaart G. Schaart Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author F. R. Pieper F. R. Pieper Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author F. C. Ramaekers F. C. Ramaekers Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author H. T. Cuypers H. T. Cuypers Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author R. M. van den Heuvel R. M. van den Heuvel Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author W. T. Vree Egberts W. T. Vree Egberts Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author G. J. van Eys G. J. van Eys Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author A. Berns A. Berns Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author H. Bloemendal H. Bloemendal Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author P. J. Krimpenfort P. J. Krimpenfort Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author G. Schaart G. Schaart Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author F. R. Pieper F. R. Pieper Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author F. C. Ramaekers F. C. Ramaekers Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author H. T. Cuypers H. T. Cuypers Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author R. M. van den Heuvel R. M. van den Heuvel Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author W. T. Vree Egberts W. T. Vree Egberts Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author G. J. van Eys G. J. van Eys Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author A. Berns A. Berns Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author H. Bloemendal H. Bloemendal Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. Search for more papers by this author Author Information P. J. Krimpenfort1, G. Schaart1, F. R. Pieper1, F. C. Ramaekers1, H. T. Cuypers1, R. M. Heuvel1, W. T. Vree Egberts1, G. J. Eys1, A. Berns1 and H. Bloemendal1 1Division of Molecular Genetics, Netherlands Cancer Institute Amsterdam. The EMBO Journal (1988)7:941-947https://doi.org/10.1002/j.1460-2075.1988.tb02899.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have introduced a hybrid gene, pVVim2, composed of the 5′ region of the hamster vimentin gene encoding the head and rod domain of vimentin and the 3′ region of the hamster desmin gene encoding the tail domain of desmin, into the germ line of mice by pronuclear injection. RNA and protein analysis of mice transgenic for this construct showed that the pVVim2 gene was expressed at high levels in a developmental and tissue-specific manner. This indicates that the vimentin-derived segment of the fusion gene contains all the regulatory elements required for vimentin-specific expression. Immunohistochemical staining of fibroblast cultures derived from the transgenic mice with antibodies specific for vimentin and desmin demonstrated that the pVVim2 protein is assembled into filaments that co-localize with the endogenous vimentin filaments. The expression of pVVim2 protein in mesenchymal cells does not interfere with the function of vimentin in these cells. Previous ArticleNext Article Volume 7Issue 41 April 1988In this issue RelatedDetailsLoading ..." @default.
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• W1606270170 title "Tissue-specific expression of a vimentin-desmin hybrid gene in transgenic mice." @default.
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