FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1606286273> ?p ?o ?g. }

• W1606286273 endingPage "7107" @default.
• W1606286273 startingPage "7102" @default.
• W1606286273 abstract "An immunoaffinity chromatographic procedure was developed to purify DNA polymerase-DNA primase complex from crude soluble extracts of yeast cells. The immunoabsorbent column is made of mouse monoclonal antibody to yeast DNA polymerase I covalently linked to Protein A-Sepharose. Purification of the complex involves binding of the complex to the immunoabsorbent column and elution with concentrated MgCl2 solutions. After rebinding to the monoclonal antibody column free primase activity is selectively eluted with a lower concentration of MgCl2. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate showed the presence of five major peptides, p180, p140, p74, p58, and p48 in the immunoaffinity-purified DNA polymerase-DNA primase complex. Free primase and free polymerase fractions obtained by fractionation on the immunoabsorbent column were analyzed on activity gels and immunoblots. These analyses showed that p180 and p140 are DNA polymerase peptides. Two polypeptides of 58 and 48 kDa co-fractionated with the free yeast DNA primase. From sucrose gradient analysis we estimate a molecular weight of 110 kDa for the native DNA primase." @default.
• W1606286273 created "2016-06-24" @default.
• W1606286273 creator A5004946680 @default.
• W1606286273 creator A5009360205 @default.
• W1606286273 creator A5021882110 @default.
• W1606286273 creator A5035404530 @default.
• W1606286273 creator A5073401476 @default.
• W1606286273 creator A5079778801 @default.
• W1606286273 date "1985-06-01" @default.
• W1606286273 modified "2023-10-02" @default.
• W1606286273 title "Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex." @default.
• W1606286273 cites W127972909 @default.
• W1606286273 cites W1485247673 @default.
• W1606286273 cites W1487826023 @default.
• W1606286273 cites W1507446938 @default.
• W1606286273 cites W1515720480 @default.
• W1606286273 cites W1524532519 @default.
• W1606286273 cites W1525276477 @default.
• W1606286273 cites W1548277098 @default.
• W1606286273 cites W1563332050 @default.
• W1606286273 cites W1579328338 @default.
• W1606286273 cites W1587694577 @default.
• W1606286273 cites W1592161081 @default.
• W1606286273 cites W1604705323 @default.
• W1606286273 cites W1656024959 @default.
• W1606286273 cites W2023865120 @default.
• W1606286273 cites W2027290354 @default.
• W1606286273 cites W2034939386 @default.
• W1606286273 cites W2038626641 @default.
• W1606286273 cites W2038886444 @default.
• W1606286273 cites W2040992944 @default.
• W1606286273 cites W2056647681 @default.
• W1606286273 cites W2067480482 @default.
• W1606286273 cites W2080149726 @default.
• W1606286273 cites W2100837269 @default.
• W1606286273 cites W2413578079 @default.
• W1606286273 cites W2573644289 @default.
• W1606286273 cites W78111501 @default.
• W1606286273 doi "https://doi.org/10.1016/s0021-9258(18)88893-7" @default.
• W1606286273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3888995" @default.
• W1606286273 hasPublicationYear "1985" @default.
• W1606286273 type Work @default.
• W1606286273 sameAs 1606286273 @default.
• W1606286273 citedByCount "112" @default.
• W1606286273 countsByYear W16062862732013 @default.
• W1606286273 countsByYear W16062862732017 @default.
• W1606286273 countsByYear W16062862732020 @default.
• W1606286273 countsByYear W16062862732021 @default.
• W1606286273 crossrefType "journal-article" @default.
• W1606286273 hasAuthorship W1606286273A5004946680 @default.
• W1606286273 hasAuthorship W1606286273A5009360205 @default.
• W1606286273 hasAuthorship W1606286273A5021882110 @default.
• W1606286273 hasAuthorship W1606286273A5035404530 @default.
• W1606286273 hasAuthorship W1606286273A5073401476 @default.
• W1606286273 hasAuthorship W1606286273A5079778801 @default.
• W1606286273 hasBestOaLocation W16062862731 @default.
• W1606286273 hasConcept C104317684 @default.
• W1606286273 hasConcept C149187182 @default.
• W1606286273 hasConcept C153911025 @default.
• W1606286273 hasConcept C156719811 @default.
• W1606286273 hasConcept C178790620 @default.
• W1606286273 hasConcept C185592680 @default.
• W1606286273 hasConcept C194830204 @default.
• W1606286273 hasConcept C2756471 @default.
• W1606286273 hasConcept C2777563447 @default.
• W1606286273 hasConcept C2779222958 @default.
• W1606286273 hasConcept C31254050 @default.
• W1606286273 hasConcept C49105822 @default.
• W1606286273 hasConcept C54355233 @default.
• W1606286273 hasConcept C552990157 @default.
• W1606286273 hasConcept C55493867 @default.
• W1606286273 hasConcept C58167604 @default.
• W1606286273 hasConcept C73573662 @default.
• W1606286273 hasConcept C82381507 @default.
• W1606286273 hasConcept C86803240 @default.
• W1606286273 hasConcept C88354546 @default.
• W1606286273 hasConceptScore W1606286273C104317684 @default.
• W1606286273 hasConceptScore W1606286273C149187182 @default.
• W1606286273 hasConceptScore W1606286273C153911025 @default.
• W1606286273 hasConceptScore W1606286273C156719811 @default.
• W1606286273 hasConceptScore W1606286273C178790620 @default.
• W1606286273 hasConceptScore W1606286273C185592680 @default.
• W1606286273 hasConceptScore W1606286273C194830204 @default.
• W1606286273 hasConceptScore W1606286273C2756471 @default.
• W1606286273 hasConceptScore W1606286273C2777563447 @default.
• W1606286273 hasConceptScore W1606286273C2779222958 @default.
• W1606286273 hasConceptScore W1606286273C31254050 @default.
• W1606286273 hasConceptScore W1606286273C49105822 @default.
• W1606286273 hasConceptScore W1606286273C54355233 @default.
• W1606286273 hasConceptScore W1606286273C552990157 @default.
• W1606286273 hasConceptScore W1606286273C55493867 @default.
• W1606286273 hasConceptScore W1606286273C58167604 @default.
• W1606286273 hasConceptScore W1606286273C73573662 @default.
• W1606286273 hasConceptScore W1606286273C82381507 @default.
• W1606286273 hasConceptScore W1606286273C86803240 @default.
• W1606286273 hasConceptScore W1606286273C88354546 @default.
• W1606286273 hasIssue "11" @default.
• W1606286273 hasLocation W16062862731 @default.

• next