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• W1606382016 abstract "Publisher Summary Antisense strategy for the prevention of the function of the target gene was first described by Belikova et al. in 1967. Since then, antisense strategy using synthetic oligodeoxynucleotides has been applied to the suppression of specific gene expression, related to tumorigenesis, growth, or development of tumors. Antisense strategy using synthetic oligodeoxynucleotides can also suppress the biological activity of the corresponding gene, serving as one of the effective therapeutic choices. These works range from a pioneer in cell-free extracts and cultured cells to more recent in vivo experiments. Because synthetic oligodeoxynucleotides have more advantages over synthetic oligoribonucleotides, the antisense technique using synthetic oligodeoxynucleotides has become a widely used method for the modulation of various gene expressions and its biological activity. This chapter describes an antisense technique using an in vitro and in vivo delivery system of lipofection and demonstrates the growth suppression of rat C6 glioma cells utilizing the antisense synthetic oligodeoxynucleotide for microtubule-associated protein (MAP) 1A mRNA with emphasis on the utility of this system." @default.
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• W1606382016 date "2000-01-01" @default.
• W1606382016 modified "2023-09-27" @default.
• W1606382016 title "In vitro and in vivo delivery of antisense oligodeoxynucleotides using lipofection: Application of antisense technique to growth suppression of experimental glioma" @default.
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• W1606382016 doi "https://doi.org/10.1016/s0076-6879(00)13022-8" @default.
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