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- W1606392034 abstract "Penetration of measles virus (Toyoshima strain) in African Green monkey kidney-derived Vero cells was studied in detail.Treatment of cell-virus complex with pH 3.0, 5 minutes' pulse was found effective to exterminate viral infectivity without leaving any disturbance on viabilities of cell and infective center. Hyperimmune antiserum of guinea pig against measles virus was not potent enough in eliminating free and cell-attached viruses from reaction mixture in a short time.Therefore, ‘penetrated virus’ is defined in this paper as a survived infective center after the low pH treatment.Penetration, thus determined, followed the first order kinetics, and its initial velocity was enhanced by centrifugal force applied to the cell-virus complex.This process was found being dependent on reaction temperature, nutritional condition of the cell, inhibited by added monoiodoacetate and sodium fluoride, not by potassium cyanide or sodium malonate.Anticellular guinea pig serum, when treated prior to viral adsorption, suppressed the penetration markedly.These findings strongly suggested the identity of the most part of this process to the well-established phagocytotic activity of cells.Trials to locate the site where specificity of viral infection being regulated are now underway." @default.
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- W1606392034 date "1967-01-01" @default.
- W1606392034 modified "2023-09-26" @default.
- W1606392034 title "PENETRATION OF MEASLES VIRUS IN VERO CELLS, ASSAY PROCEDURE AND SEVERAL BASIC PROPERTIES" @default.
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- W1606392034 doi "https://doi.org/10.2222/jsv.17.44" @default.
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