FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1606571684> ?p ?o ?g. }

• W1606571684 endingPage "4699" @default.
• W1606571684 startingPage "4693" @default.
• W1606571684 abstract "Studies have been conducted to characterize further the interaction between 125I-labeled bovine thyrotropin (TSH) and bovine thyroid plasma membranes. Sequential subcellular fractionation of thyroid homogenates yielded preparations of progressively greater specific binding activity, highest activity being found in fractions previously shown to contain predominately plasma membranes (Amir, S. M., Carraway, T.F., Kohn, L.D., and Winand, R.J. (1973) J. Biol. Chem. 248, 4092-4100). Although binding of 125I-TSH by plasma membranes was greatest at pH 6.0, studies were conducted at pH 7.45 as well as pH 6.0, and results obtained differed quantitatively, but not qualitatively. Binding was maximal at 0 degrees, 15 degrees, and 22 degrees and steady state values remained unchanged for at least 22 hours. At 37 degrees, binding was decreased by 40% at 1 hour; the loss was even greater (65%) at 50 degrees. A similar loss of binding was evident when membranes were preincubated without TSH at 37 degrees or higher and were then incubated with 125I-TSH at 0 degrees. Lineweaver-Burk analysis indicated that preincubation resulted in loss of receptor sites without change in affinity of residual receptors. Addition of Ca2+ (1 to 10 mM) to the preincubation medium prevented the effect of preincubation at 37 degrees by preserving the number of receptor sites without altering their affinity. Under similar conditions, Na+ and K+ were without protective effect. Membranes bound 45Ca2+ in a specific and saturable manner. Scatchard plots indicated a dissociatiion constant (Kd) of 9 X 10(-5) M and a capacity (n) of 54 nmol/mg of membrane protein. 45Ca2+ was also displaced from membranes by Mg2+ and Mn2+. Ca2+ had a biphasic effect on binding; low concentrations (1 to 10 muM) added to the incubation mixture stimulated binding, while higher concentrations (0.1 mM) caused inhibition. Mg2+ and Mn2+, at comparable concentrations, were also inhibitory, Na+ and K+ less so. In the case of Ca2+, both the stimulatory and inhibitory concentrations were lower than those required to achieve saturation of Ca2+-binding sites. Proteolytic enzymes (trypsin, alpha-chymotrypsin, and pronase) sharply reduced binding of 125I-TSH, owing to a decrease in receptor sites. Phospholipases A and C enhanced binding of TSH, while neuraminidase and beta-galactosidase were without measurable effect." @default.
• W1606571684 created "2016-06-24" @default.
• W1606571684 creator A5071042751 @default.
• W1606571684 creator A5091065594 @default.
• W1606571684 creator A5091189105 @default.
• W1606571684 date "1976-08-01" @default.
• W1606571684 modified "2023-09-30" @default.
• W1606571684 title "Properties of the interaction between bovine thyrotropin and bovine thyroid plasma membranes." @default.
• W1606571684 cites W1491229680 @default.
• W1606571684 cites W1503983718 @default.
• W1606571684 cites W1534479941 @default.
• W1606571684 cites W1534905276 @default.
• W1606571684 cites W1564929051 @default.
• W1606571684 cites W1654440136 @default.
• W1606571684 cites W1775749144 @default.
• W1606571684 cites W1938758335 @default.
• W1606571684 cites W1966340517 @default.
• W1606571684 cites W1983649348 @default.
• W1606571684 cites W1987220066 @default.
• W1606571684 cites W1989621958 @default.
• W1606571684 cites W1990696910 @default.
• W1606571684 cites W1994479571 @default.
• W1606571684 cites W2010969834 @default.
• W1606571684 cites W2014121187 @default.
• W1606571684 cites W2045009554 @default.
• W1606571684 cites W2081923188 @default.
• W1606571684 cites W2089840619 @default.
• W1606571684 cites W2094412020 @default.
• W1606571684 cites W2113537773 @default.
• W1606571684 cites W4242856158 @default.
• W1606571684 doi "https://doi.org/10.1016/s0021-9258(17)33258-1" @default.
• W1606571684 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/181381" @default.
• W1606571684 hasPublicationYear "1976" @default.
• W1606571684 type Work @default.
• W1606571684 sameAs 1606571684 @default.
• W1606571684 citedByCount "47" @default.
• W1606571684 crossrefType "journal-article" @default.
• W1606571684 hasAuthorship W1606571684A5071042751 @default.
• W1606571684 hasAuthorship W1606571684A5091065594 @default.
• W1606571684 hasAuthorship W1606571684A5091189105 @default.
• W1606571684 hasBestOaLocation W16065716841 @default.
• W1606571684 hasConcept C126322002 @default.
• W1606571684 hasConcept C134018914 @default.
• W1606571684 hasConcept C185592680 @default.
• W1606571684 hasConcept C41625074 @default.
• W1606571684 hasConcept C526584372 @default.
• W1606571684 hasConcept C55493867 @default.
• W1606571684 hasConcept C71924100 @default.
• W1606571684 hasConcept C86803240 @default.
• W1606571684 hasConceptScore W1606571684C126322002 @default.
• W1606571684 hasConceptScore W1606571684C134018914 @default.
• W1606571684 hasConceptScore W1606571684C185592680 @default.
• W1606571684 hasConceptScore W1606571684C41625074 @default.
• W1606571684 hasConceptScore W1606571684C526584372 @default.
• W1606571684 hasConceptScore W1606571684C55493867 @default.
• W1606571684 hasConceptScore W1606571684C71924100 @default.
• W1606571684 hasConceptScore W1606571684C86803240 @default.
• W1606571684 hasIssue "15" @default.
• W1606571684 hasLocation W16065716841 @default.
• W1606571684 hasOpenAccess W1606571684 @default.
• W1606571684 hasPrimaryLocation W16065716841 @default.
• W1606571684 hasRelatedWork W1981968121 @default.
• W1606571684 hasRelatedWork W2010645160 @default.
• W1606571684 hasRelatedWork W2011960454 @default.
• W1606571684 hasRelatedWork W2021841321 @default.
• W1606571684 hasRelatedWork W2060603500 @default.
• W1606571684 hasRelatedWork W2070295037 @default.
• W1606571684 hasRelatedWork W2088569338 @default.
• W1606571684 hasRelatedWork W2163236351 @default.
• W1606571684 hasRelatedWork W2411536504 @default.
• W1606571684 hasRelatedWork W2748952813 @default.
• W1606571684 hasVolume "251" @default.
• W1606571684 isParatext "false" @default.
• W1606571684 isRetracted "false" @default.
• W1606571684 magId "1606571684" @default.
• W1606571684 workType "article" @default.