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- W1606656891 abstract "A mouse L cell line which expresses the herpex simplex virus type 1 immediate-early polypeptides ICP4 and ICP47 was cotransfected with a cloned copy of the BglII L fragment of herpes simplex virus type 2, which includes the gene for gD, and the plasmid pSV2neo, which contains the aminoglycosyl 3'-phosphotransferase (agpt) gene conferring resistance to the antibiotic G418. A G418-resistant transformed cell line was isolated which expressed herpes simplex virus type 2 gD at higher levels than were found in infected cells. The intracellular transport and processing of gD was compared in transformed and infected cells. In the transformed Z4/6 cells gD was rapidly processed and transported to the cell surface; in contrast, the processing and cell surface appearance of gD in infected parental Z4 cells occurred at a much slower rate, and gD accumulated in nuclear membrane to a greater extent. Thus, the movement of HSV-2 gD to the cell surface in infected cells is retarded as viral glycoproteins accumulate in the nuclear envelope, probably because they interact with other viral structural components." @default.
- W1606656891 created "2016-06-24" @default.
- W1606656891 creator A5015898479 @default.
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- W1606656891 date "1985-06-01" @default.
- W1606656891 modified "2023-10-16" @default.
- W1606656891 title "Intracellular transport of herpes simplex virus gD occurs more rapidly in uninfected cells than in infected cells" @default.
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- W1606656891 doi "https://doi.org/10.1128/jvi.54.3.682-689.1985" @default.
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