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- W1606865874 abstract "Publisher Summary The Semliki forest virus (SFV) is a positive-stranded RNA virus belonging to the alphaviruses. The SFV expression system is based on a cDNA copy of the viral genome. The cDNA has been cloned into an SP6-based transcription vector in such a way that exact copies of the alphavirus RNA genome can be transcribed in vitro. The recombinant plasmid then serves as a template for in vitro synthesis of recombinant RNA. When introduced into cells, the recombinant RNA self-replicates, as it codes for its own replicase, leading to high synthesis of the heterologous protein while competing out the host protein synthesis. In the SFVC vector, the viral capsid gene is retained and the hierologist gene is cloned directly downstream of the capsid gene by polymerase chain reaction (PCR). One needs to avoid repeated freezing and thawing of the virus stock. This will reduce virus infectivity. For use in electroporation, the cells should not be passaged more than about 30 times." @default.
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- W1606865874 date "2014-07-15" @default.
- W1606865874 modified "2023-10-04" @default.
- W1606865874 title "Semliki Forest VirusExpression System" @default.
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- W1606865874 doi "https://doi.org/10.1002/9780470015902.a0002661.pub3" @default.
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