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- W1607056570 abstract "Capillary electrophoresis combined with contactless conductivity detection allows toseparate and detect the ionic species, which are neither UV absorbing nor fluorescent.This thesis focuses on the applications of this method on enzymatic reactions indifferent analytical tasks.First, the non-ionic species ethanol, glucose, ethyl acetate and ethyl butyrate weremade accessible for analysis by capillary electrophoresis via charged products orbyproducts obtained in enzymatic conversions using hexokinase, glucose oxidase,alcohol dehydrogenase and esterase. The conversion of glucose and that ofethylacetate were also successfully demonstrated on a microchip-device.Quantification of ethyl acetate, was found possible with a detection limit ofapproximately 7 μM. Then the model of urea catalyzed by urease was chosen for thestudy of the enzymatic mechanism, the effect of substrate concentration and pH valueas well as the Michaelis-Menten constant. The determination of urea in human bloodas clinical application of this enzymatic reaction was tested. 10 human blood sampleswere collected from a hospital and analyzed. The results were comparable with theestablished methods. The method was then extended to proteome analysis;identification of proteins is generally achieved through proteolytic digestion withenzymes such as pepsin and trypsin. Protein digestion with pepsin and trypsin wassuccessfully monitored by capillary electrophoresis. Minigastrin I, myoglobin,cytochrome C, human serum albumin and bovine serum album were the modelproteins digested by pepsin, cytochrome C and myoglobin were the model proteinsdigested by trypsin. Electrophoretically mediated micro-analysis (EMMA) techniqueis employed for the tryptic digestion of cytochrome C and apomyoglobin.Finally, the enantioselective hydrolysis of esters of amino acids with lipase wasmonitored. Porcine pancreas lipase was found to have a better efficiency onhydrolysis and enantioselectivity than wheat germ lipase. L-threonine methyl esterdemonstrated stronger enantioselectivity than L-serine methyl ester.Acetylcholinesterase inhibitors can be used as drug against Alzheimer disease ornerve agents. Three compounds, namely galantamine, paraoxon and Huperzine-A,were the model inhibitors to study the behavior and kinetics of the inhibitors. Thevalues of IC50 were obtained through graphical plot. Their dependence on the timecourse was monitored and graphically illustrated." @default.
- W1607056570 created "2016-06-24" @default.
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- W1607056570 date "2009-01-01" @default.
- W1607056570 modified "2023-09-27" @default.
- W1607056570 title "Monitoring of enzymatic reactions using capillary electrophoresis with conductivity detection" @default.
- W1607056570 doi "https://doi.org/10.5451/unibas-004820768" @default.
- W1607056570 hasPublicationYear "2009" @default.
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