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- W1607172570 abstract "A reproducible in vitro test was developed to quantitatively study the adhesion of human eosinophils to Wuchereria bancrofti infective larvae. Eosinophils, regardless of the donor, selectively adhered to the larvae in the presence of immune serum. The reaction reached a maximum by 90 minutes at room temperature and remained unchanged up to 6 hours. The adherent eosinophils, however, did not induce any apparent morphologic change in the larvae.The phenomenon appeared to require, primarily, IgG anti-larval antibodies. Heat-inactivation of the serum did not prevent the reaction from occurring, although addition of fresh normal serum enhanced the intensity of adhesion.Maximal adhesion of eosinophils was obtained when the larvae were viable and in the presence of immune serum and fresh normal serum during incubation with the leucocytes.Normal serum was found to induce this adhesion reaction. The responsible factor could be removed by absorption of normal serum with cotton. However, this procedure had no effect on the reactivity of sera from filariasis cases.The reaction was almost totally inhibited by EDTA and citrate. The anti-inflammatory steroid, betamethasone, had a moderately inhibitory effect. An unexplained finding was an enhancing effect on the reaction when histamine was added to non-reactive normal serum." @default.
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- W1607172570 title "In vitro adhesion of eosinophils to infective larvae of Wuchereria bancrofti." @default.
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