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- W1607474583 abstract "Membrane-bound UDP-glucose:sterol [beta]-D-glucosyltransferase (UDPG-SGTase) catalyzes the formation of steryl glucosides from UDP-glucose and free sterols. This enzyme was purified from etiolated oat shoots (Avena sativa L. cv Alfred) in five steps. UDPG-SGTase was solubilized from a microsomal fraction with the detergent n-octyl-[beta]-D-thioglucopyranoside and then extracted into diethyl ether. Subsequent removal of the organic solvent, resolubilization with an aqueous buffer, and two column chromatographic steps on Q-Sepharose and Blue Sepharose resulted in a 12,500-fold overall purification. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the final preparation revealed a 56-kD protein band, the intensity of which correlated with enzyme activity in the respective fractions. Polyclonal antibodies raised against this 56-kD protein did not inhibit enzyme activity but specifically bound to the native UDPG-SGTase. These results suggest that the 56-kD protein represents the UDPG-SGTase. The purified enzyme was specific for UDP-glucose (Km = 34 [mu]M), for which UDP was a competitive inhibitor (inhibitor constant = 47 [mu]M). In contrast to the specificity with regard to the glycosyl donor, UDPG-SGTase utilized all tested sterol acceptors, such as [beta]-sitosterol, cholesterol, stigmasterol, and ergosterol." @default.
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- W1607474583 date "1994-08-01" @default.
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- W1607474583 title "Purification of a Membrane-Bound UDP-Glucose:Sterol [beta]-D-Glucosyltransferase Based on Its Solubility in Diethyl Ether" @default.
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- W1607474583 doi "https://doi.org/10.1104/pp.105.4.1067" @default.
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