Matches in SemOpenAlex for { <https://semopenalex.org/work/W160815419> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W160815419 endingPage "312" @default.
- W160815419 startingPage "309" @default.
- W160815419 abstract "This chapter focuses on the calcium overlay assay, which is a general metal ion binding assay. The method has been used to identify calcium-binding proteins that contain EF hands as well as other types of calcium-binding proteins transferred to nitrocellulose from either one- or two dimensional gels. The procedure can be performed at room temperature within 24 hour and can detect as little as 20 pmole of calcium binding sites. The method does not reveal any noticeable differences in calcium binding between membranes that had been dried before probing and membranes that had been used directly after blotting. Fresh imidazole solution should be used while performing this method. The membrane between sheets of filter paper should not be dried as this procedure gives severe background problems." @default.
- W160815419 created "2016-06-24" @default.
- W160815419 creator A5007234546 @default.
- W160815419 creator A5070313094 @default.
- W160815419 date "1994-01-01" @default.
- W160815419 modified "2023-09-26" @default.
- W160815419 title "Calcium Overlay Assay" @default.
- W160815419 cites W1608114043 @default.
- W160815419 cites W2013742184 @default.
- W160815419 cites W2056001186 @default.
- W160815419 cites W2140807560 @default.
- W160815419 cites W2172049940 @default.
- W160815419 doi "https://doi.org/10.1016/b978-0-12-164717-9.50044-1" @default.
- W160815419 hasPublicationYear "1994" @default.
- W160815419 type Work @default.
- W160815419 sameAs 160815419 @default.
- W160815419 citedByCount "1" @default.
- W160815419 crossrefType "book-chapter" @default.
- W160815419 hasAuthorship W160815419A5007234546 @default.
- W160815419 hasAuthorship W160815419A5070313094 @default.
- W160815419 hasConcept C104317684 @default.
- W160815419 hasConcept C107824862 @default.
- W160815419 hasConcept C12554922 @default.
- W160815419 hasConcept C170493617 @default.
- W160815419 hasConcept C178790620 @default.
- W160815419 hasConcept C185592680 @default.
- W160815419 hasConcept C2778954975 @default.
- W160815419 hasConcept C2780874372 @default.
- W160815419 hasConcept C41625074 @default.
- W160815419 hasConcept C43617362 @default.
- W160815419 hasConcept C519063684 @default.
- W160815419 hasConcept C55493867 @default.
- W160815419 hasConcept C85196483 @default.
- W160815419 hasConcept C85265743 @default.
- W160815419 hasConcept C86803240 @default.
- W160815419 hasConceptScore W160815419C104317684 @default.
- W160815419 hasConceptScore W160815419C107824862 @default.
- W160815419 hasConceptScore W160815419C12554922 @default.
- W160815419 hasConceptScore W160815419C170493617 @default.
- W160815419 hasConceptScore W160815419C178790620 @default.
- W160815419 hasConceptScore W160815419C185592680 @default.
- W160815419 hasConceptScore W160815419C2778954975 @default.
- W160815419 hasConceptScore W160815419C2780874372 @default.
- W160815419 hasConceptScore W160815419C41625074 @default.
- W160815419 hasConceptScore W160815419C43617362 @default.
- W160815419 hasConceptScore W160815419C519063684 @default.
- W160815419 hasConceptScore W160815419C55493867 @default.
- W160815419 hasConceptScore W160815419C85196483 @default.
- W160815419 hasConceptScore W160815419C85265743 @default.
- W160815419 hasConceptScore W160815419C86803240 @default.
- W160815419 hasLocation W1608154191 @default.
- W160815419 hasOpenAccess W160815419 @default.
- W160815419 hasPrimaryLocation W1608154191 @default.
- W160815419 isParatext "false" @default.
- W160815419 isRetracted "false" @default.
- W160815419 magId "160815419" @default.
- W160815419 workType "book-chapter" @default.