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• W160828296 abstract "This chapter focuses on the three-dimensional molecular structure of the reaction center of the purple bacterium Rhodopseudomonas viridis. In an experiment described in the chapter, three-dimensional crystals of the reaction center (RC) of Rhodopseudomonas viridis were obtained from RCs solubilized with the detergent N,N-dimethyl dodecylamine-N-oxide by using the sitting-drop vapor-diffusion technique with ammonium sulfate as the precipitant. Addition of the small amphiphile heptane-1,2,3-triol to the protein solution was essential for crystallization. With synchrotron radiation as the X-ray source, the crystals diffracted X-rays to at least 2.3 Å resolution. So even on photographic film, a substantial portion of a diffraction data set could be collected from one crystal. The distribution of the detergent in the crystal was determined in a low-resolution neutron diffraction study. The elucidation of the three-dimensional structure of the RC of Rhodopseudomonas viridis significantly influenced the identification of the photosystem II (PSII) RC of green plants and cyanobacteria. The three-dimensional RC structures revealed that the sequence homologies include key amino acids. These and other findings strengthen the hypothesis that the RCs of purple bacteria and of PSII are related structurally." @default.
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• W160828296 date "1993-01-01" @default.
• W160828296 modified "2023-09-26" @default.
• W160828296 title "Three-Dimensional Structure of the Reaction Center of Rhodopseudomonas viridis" @default.
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• W160828296 doi "https://doi.org/10.1016/b978-0-12-208662-5.50022-0" @default.
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