FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1608665731> ?p ?o ?g. }

• W1608665731 endingPage "4198" @default.
• W1608665731 startingPage "4189" @default.
• W1608665731 abstract "We have examined the factors which influence the expression of a major 2.7-kilobase (kb) early transcript encoded by the long repeat of the human cytomegalovirus (HCMV) strain AD169 genome. Previously, by deletion analysis, we determined that the promoter for this early RNA consisted of multiple cis-acting elements (Klucher et al., J. Virol. 63:5334-5343, 1989). Using extracts prepared from HeLa cells as well as from infected and uninfected foreskin fibroblasts, we also obtained evidence for the interaction of a cellular factor with one of these elements. In this study, we have further defined the specificity and functional importance of this binding. On the basis of DNase I footprinting and methylation interference assays, we localized the site of interaction to a region (nucleotides -113 to -106 relative to the mRNA start site) which contains homology to the binding site for the adenovirus major late transcription factor (MLTF), also referred to as the upstream stimulatory factor (USF). The contact points of binding between the cellular factor and the guanine residues within this segment were consistent with the pattern of binding for USF/MLTF. Additionally, by using oligonucleotides containing the binding sites for USF/MLTF from the adenovirus major late promoter and the HCMV 2.7-kb RNA promoter as competitors in gel retardation assays, we were able to show that USF/MLTF bound to the two promoters with similar affinity. Correlation of the binding activity with in vivo functional importance was provided by mutagenesis and transient-expression assays. A point mutation within the HCMV USF/MLTF site lowered the affinity of binding 5- to 10-fold and decreased the inducible activity of the HCMV 2.7-kb RNA promoter by approximately 50%. Furthermore, the addition of the HCMV USF/MLTF site to a minimal 2.7-kb RNA promoter containing only the TATA sequence resulted in an increase in HCMV inducible transcriptional activity of 6- to 20-fold. However, the HCMV USF/MLTF site could not functionally substitute for the TATA sequence. These studies further support the idea that for maximal response to the HCMV infection, the 2.7-kb RNA promoter requires multiple cis-acting sequences, two of which include the binding sites for USF/MLTF and TFIID." @default.
• W1608665731 created "2016-06-24" @default.
• W1608665731 creator A5033878540 @default.
• W1608665731 creator A5052328406 @default.
• W1608665731 date "1990-09-01" @default.
• W1608665731 modified "2023-10-16" @default.
• W1608665731 title "The human cytomegalovirus 2.7-kilobase RNA promoter contains a functional binding site for the adenovirus major late transcription factor" @default.
• W1608665731 cites W142428048 @default.
• W1608665731 cites W1441914411 @default.
• W1608665731 cites W1492794412 @default.
• W1608665731 cites W1496664311 @default.
• W1608665731 cites W1501688137 @default.
• W1608665731 cites W150716091 @default.
• W1608665731 cites W1539265613 @default.
• W1608665731 cites W1558516755 @default.
• W1608665731 cites W1560711427 @default.
• W1608665731 cites W1563038486 @default.
• W1608665731 cites W1579186703 @default.
• W1608665731 cites W1601164489 @default.
• W1608665731 cites W1605790582 @default.
• W1608665731 cites W1797852794 @default.
• W1608665731 cites W1816943591 @default.
• W1608665731 cites W1852665292 @default.
• W1608665731 cites W1855074355 @default.
• W1608665731 cites W1869386653 @default.
• W1608665731 cites W1885968493 @default.
• W1608665731 cites W1887844619 @default.
• W1608665731 cites W1908567109 @default.
• W1608665731 cites W1916260177 @default.
• W1608665731 cites W1933815796 @default.
• W1608665731 cites W1952117209 @default.
• W1608665731 cites W1953137162 @default.
• W1608665731 cites W1960156471 @default.
• W1608665731 cites W1967294243 @default.
• W1608665731 cites W1982692581 @default.
• W1608665731 cites W1982726429 @default.
• W1608665731 cites W1986291674 @default.
• W1608665731 cites W1987876212 @default.
• W1608665731 cites W1988762312 @default.
• W1608665731 cites W1993243592 @default.
• W1608665731 cites W2000618235 @default.
• W1608665731 cites W2026460545 @default.
• W1608665731 cites W2026550263 @default.
• W1608665731 cites W2027370571 @default.
• W1608665731 cites W2037310000 @default.
• W1608665731 cites W2038940735 @default.
• W1608665731 cites W2040193953 @default.
• W1608665731 cites W2042976701 @default.
• W1608665731 cites W2056967020 @default.
• W1608665731 cites W2090554769 @default.
• W1608665731 cites W2101290459 @default.
• W1608665731 cites W2118018893 @default.
• W1608665731 cites W2125045982 @default.
• W1608665731 cites W2128635872 @default.
• W1608665731 cites W2134764060 @default.
• W1608665731 cites W2138270253 @default.
• W1608665731 cites W2144206181 @default.
• W1608665731 cites W2144308565 @default.
• W1608665731 cites W2166613267 @default.
• W1608665731 cites W2166801871 @default.
• W1608665731 cites W2167559681 @default.
• W1608665731 cites W223357076 @default.
• W1608665731 cites W2290087437 @default.
• W1608665731 cites W2311220916 @default.
• W1608665731 cites W2334595670 @default.
• W1608665731 cites W97754571 @default.
• W1608665731 doi "https://doi.org/10.1128/jvi.64.9.4189-4198.1990" @default.
• W1608665731 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/247883" @default.
• W1608665731 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2166813" @default.
• W1608665731 hasPublicationYear "1990" @default.
• W1608665731 type Work @default.
• W1608665731 sameAs 1608665731 @default.
• W1608665731 citedByCount "39" @default.
• W1608665731 countsByYear W16086657312020 @default.
• W1608665731 crossrefType "journal-article" @default.
• W1608665731 hasAuthorship W1608665731A5033878540 @default.
• W1608665731 hasAuthorship W1608665731A5052328406 @default.
• W1608665731 hasBestOaLocation W16086657311 @default.
• W1608665731 hasConcept C101762097 @default.
• W1608665731 hasConcept C104317684 @default.
• W1608665731 hasConcept C107824862 @default.
• W1608665731 hasConcept C138885662 @default.
• W1608665731 hasConcept C150194340 @default.
• W1608665731 hasConcept C153911025 @default.
• W1608665731 hasConcept C156719811 @default.
• W1608665731 hasConcept C179926584 @default.
• W1608665731 hasConcept C2776294106 @default.
• W1608665731 hasConcept C3662595 @default.
• W1608665731 hasConcept C41895202 @default.
• W1608665731 hasConcept C54355233 @default.
• W1608665731 hasConcept C65888428 @default.
• W1608665731 hasConcept C67705224 @default.
• W1608665731 hasConcept C86339819 @default.
• W1608665731 hasConcept C86803240 @default.
• W1608665731 hasConceptScore W1608665731C101762097 @default.
• W1608665731 hasConceptScore W1608665731C104317684 @default.
• W1608665731 hasConceptScore W1608665731C107824862 @default.
• W1608665731 hasConceptScore W1608665731C138885662 @default.

• next