Matches in SemOpenAlex for { <https://semopenalex.org/work/W1609549306> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1609549306 abstract "Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregating and identifying Leptospira serovars. The method has potential application in investigating the molecular epidemiology of Leptospira.The propose of this study was genomic identification of pathogenic Leptospires in Iran by MLVA.Leptospira serovars were obtained from National Reference Laboratory of Leptospira at Razi Vaccine and Serum Research Institute, Karaj, Iran. Serovars were cultured into the liquid EMJH medium and incubated at 28˚C for 7 days. DNA of serovars was extracted using the phenol-chloroform method. PCR was performed with 5 selected variable number tandem repeat analysis (VNTR) loci. The amplified products were analyzed by agarose gel electrophoresis. The size of the amplified products was estimated by 100 bp ladder and sequencing analysis.The saprophytic serovar showed no amplified fragments. PCR products in all pathogenic serovars were observed. The 12 reference serovars used for the development of technique displayed distinct patterns.Results showed that MLVA technique with its range of polymorphism is a good marker for identification of pathogenic serovars. Some VNTR loci are more powerful than the other ones with regard to differentiation. Serovars from the same geographical area have more genetic similarity than same serovars from different places. MLVA is a suitable technique for epidemiological survey." @default.
- W1609549306 created "2016-06-24" @default.
- W1609549306 creator A5010071280 @default.
- W1609549306 creator A5017260420 @default.
- W1609549306 creator A5049750031 @default.
- W1609549306 creator A5056121031 @default.
- W1609549306 creator A5065965083 @default.
- W1609549306 creator A5082172253 @default.
- W1609549306 date "2015-10-18" @default.
- W1609549306 modified "2023-09-26" @default.
- W1609549306 title "Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis" @default.
- W1609549306 cites W1561322379 @default.
- W1609549306 cites W1898052180 @default.
- W1609549306 cites W1920942649 @default.
- W1609549306 cites W1951485283 @default.
- W1609549306 cites W1983748120 @default.
- W1609549306 cites W1986573094 @default.
- W1609549306 cites W1986882903 @default.
- W1609549306 cites W2024751292 @default.
- W1609549306 cites W2098653614 @default.
- W1609549306 cites W2111828493 @default.
- W1609549306 cites W2136919933 @default.
- W1609549306 cites W2140755388 @default.
- W1609549306 cites W2165460578 @default.
- W1609549306 cites W4211105424 @default.
- W1609549306 doi "https://doi.org/10.5812/jjm.22819" @default.
- W1609549306 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4641467" @default.
- W1609549306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26568805" @default.
- W1609549306 hasPublicationYear "2015" @default.
- W1609549306 type Work @default.
- W1609549306 sameAs 1609549306 @default.
- W1609549306 citedByCount "1" @default.
- W1609549306 countsByYear W16095493062022 @default.
- W1609549306 crossrefType "journal-article" @default.
- W1609549306 hasAuthorship W1609549306A5010071280 @default.
- W1609549306 hasAuthorship W1609549306A5017260420 @default.
- W1609549306 hasAuthorship W1609549306A5049750031 @default.
- W1609549306 hasAuthorship W1609549306A5056121031 @default.
- W1609549306 hasAuthorship W1609549306A5065965083 @default.
- W1609549306 hasAuthorship W1609549306A5082172253 @default.
- W1609549306 hasBestOaLocation W16095493062 @default.
- W1609549306 hasConcept C10389963 @default.
- W1609549306 hasConcept C104317684 @default.
- W1609549306 hasConcept C135763542 @default.
- W1609549306 hasConcept C141231307 @default.
- W1609549306 hasConcept C159047783 @default.
- W1609549306 hasConcept C180754005 @default.
- W1609549306 hasConcept C189036908 @default.
- W1609549306 hasConcept C27149982 @default.
- W1609549306 hasConcept C2776026663 @default.
- W1609549306 hasConcept C2778033228 @default.
- W1609549306 hasConcept C2780542935 @default.
- W1609549306 hasConcept C28245876 @default.
- W1609549306 hasConcept C54355233 @default.
- W1609549306 hasConcept C61320498 @default.
- W1609549306 hasConcept C70721500 @default.
- W1609549306 hasConcept C86803240 @default.
- W1609549306 hasConcept C89423630 @default.
- W1609549306 hasConcept C93720030 @default.
- W1609549306 hasConceptScore W1609549306C10389963 @default.
- W1609549306 hasConceptScore W1609549306C104317684 @default.
- W1609549306 hasConceptScore W1609549306C135763542 @default.
- W1609549306 hasConceptScore W1609549306C141231307 @default.
- W1609549306 hasConceptScore W1609549306C159047783 @default.
- W1609549306 hasConceptScore W1609549306C180754005 @default.
- W1609549306 hasConceptScore W1609549306C189036908 @default.
- W1609549306 hasConceptScore W1609549306C27149982 @default.
- W1609549306 hasConceptScore W1609549306C2776026663 @default.
- W1609549306 hasConceptScore W1609549306C2778033228 @default.
- W1609549306 hasConceptScore W1609549306C2780542935 @default.
- W1609549306 hasConceptScore W1609549306C28245876 @default.
- W1609549306 hasConceptScore W1609549306C54355233 @default.
- W1609549306 hasConceptScore W1609549306C61320498 @default.
- W1609549306 hasConceptScore W1609549306C70721500 @default.
- W1609549306 hasConceptScore W1609549306C86803240 @default.
- W1609549306 hasConceptScore W1609549306C89423630 @default.
- W1609549306 hasConceptScore W1609549306C93720030 @default.
- W1609549306 hasIssue "10" @default.
- W1609549306 hasLocation W16095493061 @default.
- W1609549306 hasLocation W16095493062 @default.
- W1609549306 hasLocation W16095493063 @default.
- W1609549306 hasLocation W16095493064 @default.
- W1609549306 hasOpenAccess W1609549306 @default.
- W1609549306 hasPrimaryLocation W16095493061 @default.
- W1609549306 hasRelatedWork W1523794008 @default.
- W1609549306 hasRelatedWork W1609549306 @default.
- W1609549306 hasRelatedWork W1947571809 @default.
- W1609549306 hasRelatedWork W2024751292 @default.
- W1609549306 hasRelatedWork W2098653614 @default.
- W1609549306 hasRelatedWork W2163186636 @default.
- W1609549306 hasRelatedWork W2325380700 @default.
- W1609549306 hasRelatedWork W2886171398 @default.
- W1609549306 hasRelatedWork W2930698185 @default.
- W1609549306 hasRelatedWork W3046707680 @default.
- W1609549306 hasVolume "8" @default.
- W1609549306 isParatext "false" @default.
- W1609549306 isRetracted "false" @default.
- W1609549306 magId "1609549306" @default.
- W1609549306 workType "article" @default.