Matches in SemOpenAlex for { <https://semopenalex.org/work/W1609933325> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W1609933325 endingPage "8261" @default.
- W1609933325 startingPage "8257" @default.
- W1609933325 abstract "Adenosine diphosphopyridoxal, the affinity labeling reagent specific for a lysyl residue in the nucleotide-binding site of several enzymes (Tagaya, M., and Fukui, T. (1986) Biochemistry 25, 2958-2964; Tamura, J. K., Rakov, R. D., and Cross R. L. (1986) J. Biol. Chem. 261, 4126-4133) was applied to adenylate kinase from rabbit muscle. Incubation of the enzyme with a low concentration of the reagent at 25 degrees C for 20 min followed by reduction by sodium borohydride resulted in rapid inactivation of the enzyme. Extrapolation to 100% loss of enzyme activity gave a value of 1.0 mol of the reagent per mol of enzyme. ADP, ATP, and MgATP almost completely protected the enzyme from inactivation, whereas AMP offered little retardation of the inactivation. Dilution of the inactivated enzyme which had not been treated with the reducing reagent led to restoration of enzyme activity. This reactivation was accelerated by ATP but not by AMP. Structural study of the labeled peptide showed that Lys21 is exclusively labeled by adenosine diphosphopyridoxal. These results suggest that the epsilon-amino group of Lys21 is located in the ATP-binding site of the enzyme, more specifically at or close to the subsite for the gamma-phosphate of the nucleotide." @default.
- W1609933325 created "2016-06-24" @default.
- W1609933325 creator A5027831160 @default.
- W1609933325 creator A5043738258 @default.
- W1609933325 creator A5060951021 @default.
- W1609933325 date "1987-06-01" @default.
- W1609933325 modified "2023-09-28" @default.
- W1609933325 title "Affinity labeling of adenylate kinase with adenosine diphosphopyridoxal. Presence of Lys21 in the ATP-binding site." @default.
- W1609933325 cites W1035097234 @default.
- W1609933325 cites W124504601 @default.
- W1609933325 cites W1489140179 @default.
- W1609933325 cites W1495821170 @default.
- W1609933325 cites W1544015573 @default.
- W1609933325 cites W1571793851 @default.
- W1609933325 cites W1599584736 @default.
- W1609933325 cites W1899186545 @default.
- W1609933325 cites W1970709570 @default.
- W1609933325 cites W1974303276 @default.
- W1609933325 cites W1981785776 @default.
- W1609933325 cites W1995064686 @default.
- W1609933325 cites W1997497266 @default.
- W1609933325 cites W2003214003 @default.
- W1609933325 cites W2012128953 @default.
- W1609933325 cites W2076805518 @default.
- W1609933325 cites W2079229201 @default.
- W1609933325 cites W2086494981 @default.
- W1609933325 cites W2146451559 @default.
- W1609933325 cites W2405321399 @default.
- W1609933325 cites W75821760 @default.
- W1609933325 doi "https://doi.org/10.1016/s0021-9258(18)47557-6" @default.
- W1609933325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3036805" @default.
- W1609933325 hasPublicationYear "1987" @default.
- W1609933325 type Work @default.
- W1609933325 sameAs 1609933325 @default.
- W1609933325 citedByCount "61" @default.
- W1609933325 crossrefType "journal-article" @default.
- W1609933325 hasAuthorship W1609933325A5027831160 @default.
- W1609933325 hasAuthorship W1609933325A5043738258 @default.
- W1609933325 hasAuthorship W1609933325A5060951021 @default.
- W1609933325 hasBestOaLocation W16099333251 @default.
- W1609933325 hasConcept C107824862 @default.
- W1609933325 hasConcept C180526460 @default.
- W1609933325 hasConcept C181199279 @default.
- W1609933325 hasConcept C185592680 @default.
- W1609933325 hasConcept C2776991684 @default.
- W1609933325 hasConcept C2779564974 @default.
- W1609933325 hasConcept C55493867 @default.
- W1609933325 hasConceptScore W1609933325C107824862 @default.
- W1609933325 hasConceptScore W1609933325C180526460 @default.
- W1609933325 hasConceptScore W1609933325C181199279 @default.
- W1609933325 hasConceptScore W1609933325C185592680 @default.
- W1609933325 hasConceptScore W1609933325C2776991684 @default.
- W1609933325 hasConceptScore W1609933325C2779564974 @default.
- W1609933325 hasConceptScore W1609933325C55493867 @default.
- W1609933325 hasIssue "17" @default.
- W1609933325 hasLocation W16099333251 @default.
- W1609933325 hasOpenAccess W1609933325 @default.
- W1609933325 hasPrimaryLocation W16099333251 @default.
- W1609933325 hasRelatedWork W1521585623 @default.
- W1609933325 hasRelatedWork W1581015106 @default.
- W1609933325 hasRelatedWork W1591629722 @default.
- W1609933325 hasRelatedWork W1970545225 @default.
- W1609933325 hasRelatedWork W1990923594 @default.
- W1609933325 hasRelatedWork W2040741921 @default.
- W1609933325 hasRelatedWork W2045499324 @default.
- W1609933325 hasRelatedWork W2948036721 @default.
- W1609933325 hasRelatedWork W331397919 @default.
- W1609933325 hasRelatedWork W2006761212 @default.
- W1609933325 hasVolume "262" @default.
- W1609933325 isParatext "false" @default.
- W1609933325 isRetracted "false" @default.
- W1609933325 magId "1609933325" @default.
- W1609933325 workType "article" @default.