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- W1610015150 abstract "This chapter discusses the magnetic solid-phase enzyme immunoassay for the quantitation of antigens and antibodies. The heterogeneous enzyme immunoassays make use of the antigen or antibody immobilized on a solid phase. Immobilization can be performed either by passive adsorption or by covalent binding. A large number of solid phases, such as polypropylene, polyvinyl, polycarbonate, aminoalkylsilyl glass, and silicone rubber, have been used for the passive adsorption of antigen or antibodies. The anti-IgE antibodies were isolated from whole antiserum, by using IgE myeloma protein, covalently coupled to glutaraldehyde-activated UI-trogel. The γ-globulin fraction of the absorbed antiserum was prepared by precipitation with ammonium sulfate at 40% saturation. The antiserum and its globulin or antibody fractions were divided into small volumes and kept frozen until used. When very small amounts of IgE have to be quantitated, fluorogenic substrates must be used for the determination of β-galactosidase activity. The 4-methylumbelliferyl-β-D-galactopyranoside has proved to be a good fluorogenic substrate for β-galactosidase. The total human IgE quantitation, by the bridged avidin-biotin technique, is also discussed in the chapter." @default.
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- W1610015150 date "1981-01-01" @default.
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- W1610015150 title "[33] Magnetic solid-phase enzyme immunoassay for the quantitation of antigens and antibodies: Application to human immunoglobulin E" @default.
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- W1610015150 doi "https://doi.org/10.1016/0076-6879(81)73086-6" @default.
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