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- W1610778995 abstract "Abstract Polylysyl transfer ribonucleic acid, free of lysyl-tRNA, has been isolated and its characteristics in an Escherichia coli polypeptide-synthesizing system in vitro have been studied. Ribosomes obtained from frozen E. coli cells were washed in 0.5 m NH4Cl and assayed at MgCl2 concentrations of at least 0.01 m. Binding of this peptidyl-tRNA to ribosomes occurs in the presence of templates containing 3 or more adenylic acid residues. p-Chloromercuriphenylsulfonate or lysyl-tRNA prevents this attachment. Binding occurs most effectively when both 30 S and 50 S ribosomal subparticles are present. Unlike lysyl-tRNA, bound polylysyl-tRNA reacts with puromycin in the absence of added GTP and supernatant factors. The product of this reaction was shown to be polylysylpuromycin. Under analogous conditions, a limited incorporation of lysine into bound polylysyl-tRNA can be shown. In this limited addition reaction, a polyadenylate template longer than three nucleotides is required. Chlortetracycline is a strong inhibitor of the limited addition of lysine to polylysyl-tRNA, but is without effect on the cleavage of polylysyl-tRNA by puromycin. Chloramphenicol, on the other hand, inhibits the reaction between polylysyl-tRNA and puromycin, but has little influence on the limited addition reaction. Gougerotin, a puromycin-like antibiotic, inhibits the puromycin reaction. Phenylalanyl-tRNA reacts poorly with puromycin in the presence of ribosomes and oligouridylate, whereas N-acetylphenylalanyl-tRNA is cleaved extensively, forming N-acetylphenylalanylpuromycin. Phenyllactyl-tRNA, although capable of binding to ribosomes, displays no detectable reactivity with puromycin. Chloramphenicol completely inhibits the cleavage of N-acetylphenylalanyl-tRNA by puromycin." @default.
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- W1610778995 date "1967-12-01" @default.
- W1610778995 modified "2023-09-30" @default.
- W1610778995 title "Reaction of Ribosome-bound Peptidyl Transfer Ribonucleic Acid with Aminoacyl Transfer Ribonucleic Acid or Puromycin" @default.
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- W1610778995 doi "https://doi.org/10.1016/s0021-9258(18)99394-4" @default.
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