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• W1612134376 abstract "The chapter attempts to bring together some examples of past and present day research on genetic engineering in the narrow context of recombinant DNA. Genetic recombination, in the conventional sense, consists in the breakage and rejoining of DNA molecules and is of fundamental importance to living organisms. The chapter explores the restriction enzymes that are defined as a class of enzymes involved in restriction-modification systems in bacteria recognized initially by their effects on bacteriophage growth in certain strains of Escherichia coli. When a DNA molecule enters a bacterial cell the restriction endonuclease “inspects” the molecule and if it contains the appropriate sequence along its length it will cleave the molecule. The modification methylase serves to protect the DNA of the bacterial cell from digestion by its own restriction enzyme and will similarly protect any DNA synthesized in that cell. The chapter describes two general methods for joining DNA molecules end-to-end. General methods for inserting specific DNA sequences into cloning vehicles are also discussed. Although the expression of eukaryotic DNA in prokaryotic cells provides the most dramatic use of in vitro recombination and cloning to cross genetic barriers, the well defined and genetically isolated Gram-negative and Gram-positive prokaryotes provide an interesting system in the replication, regulation, and expression of cloned genes to be studied." @default.
• W1612134376 created "2016-06-24" @default.
• W1612134376 creator A5022725133 @default.
• W1612134376 date "1979-01-01" @default.
• W1612134376 modified "2023-09-25" @default.
• W1612134376 title "Aspects of Genetic Engineering in Micro-Organisms" @default.
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