Matches in SemOpenAlex for { <https://semopenalex.org/work/W1612935534> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1612935534 endingPage "680" @default.
- W1612935534 startingPage "668" @default.
- W1612935534 abstract "This chapter covers the description of the procedures for the purification and the phosphorylation of the Kdp-ATPase. The Kdp-ATPase is one of at least two systems involved in potassium (K+) transport in Escherichia coli. The Kdp complex consists of three polypeptides located in the cytoplasmic membrane and is responsible for the K+-stimulated ATPase activity, associated with everted membrane vesicles. The formation of a phosphorylated intermediate during ATP hydrolysis suggests a functional homology to other eukaryotic ion transport ATPases. Frozen cells are thawed, washed once, and disrupted by a single passage through a Ribi cell fractionator (Sorvall). In the first step of the purification procedure, the everted vesicles are washed twice with lowionic-strength buffer, to remove peripherally membrane-associated proteins. The protein yield and specific activities of the Kdp-ATPase at the individual purification steps are summarized in the chapter. The washing of membranes with EDTA at low ionic strength proved to be an efficient initial step, removing about 40% of the peripheral or adsorbed membrane proteins. Under solubilization conditions, Aminoxid WS 35 extracted 50% of the residual membrane-bound proteins." @default.
- W1612935534 created "2016-06-24" @default.
- W1612935534 creator A5017273993 @default.
- W1612935534 creator A5025003953 @default.
- W1612935534 creator A5032705825 @default.
- W1612935534 date "1988-01-01" @default.
- W1612935534 modified "2023-10-14" @default.
- W1612935534 title "[52] K+-ATPase from Escherichia coli: Isolation and characterization" @default.
- W1612935534 cites W1204463526 @default.
- W1612935534 cites W1545073393 @default.
- W1612935534 cites W1547687275 @default.
- W1612935534 cites W1553379514 @default.
- W1612935534 cites W1557985843 @default.
- W1612935534 cites W1574972316 @default.
- W1612935534 cites W1578508637 @default.
- W1612935534 cites W1579123093 @default.
- W1612935534 cites W1604613452 @default.
- W1612935534 cites W1625660792 @default.
- W1612935534 cites W1775749144 @default.
- W1612935534 cites W1977475313 @default.
- W1612935534 cites W1992838345 @default.
- W1612935534 cites W2014281089 @default.
- W1612935534 cites W2018925944 @default.
- W1612935534 cites W2019934106 @default.
- W1612935534 cites W203636523 @default.
- W1612935534 cites W2052828422 @default.
- W1612935534 cites W2056097976 @default.
- W1612935534 cites W2064773838 @default.
- W1612935534 cites W2078737422 @default.
- W1612935534 cites W2101848104 @default.
- W1612935534 cites W2121745384 @default.
- W1612935534 cites W2153435210 @default.
- W1612935534 cites W2411798791 @default.
- W1612935534 cites W4293247451 @default.
- W1612935534 doi "https://doi.org/10.1016/0076-6879(88)57114-8" @default.
- W1612935534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2976471" @default.
- W1612935534 hasPublicationYear "1988" @default.
- W1612935534 type Work @default.
- W1612935534 sameAs 1612935534 @default.
- W1612935534 citedByCount "18" @default.
- W1612935534 crossrefType "book-chapter" @default.
- W1612935534 hasAuthorship W1612935534A5017273993 @default.
- W1612935534 hasAuthorship W1612935534A5025003953 @default.
- W1612935534 hasAuthorship W1612935534A5032705825 @default.
- W1612935534 hasConcept C104317684 @default.
- W1612935534 hasConcept C12554922 @default.
- W1612935534 hasConcept C130316041 @default.
- W1612935534 hasConcept C141315368 @default.
- W1612935534 hasConcept C181199279 @default.
- W1612935534 hasConcept C185592680 @default.
- W1612935534 hasConcept C190062978 @default.
- W1612935534 hasConcept C23265538 @default.
- W1612935534 hasConcept C41625074 @default.
- W1612935534 hasConcept C43617362 @default.
- W1612935534 hasConcept C547475151 @default.
- W1612935534 hasConcept C55493867 @default.
- W1612935534 hasConcept C86803240 @default.
- W1612935534 hasConcept C94412978 @default.
- W1612935534 hasConceptScore W1612935534C104317684 @default.
- W1612935534 hasConceptScore W1612935534C12554922 @default.
- W1612935534 hasConceptScore W1612935534C130316041 @default.
- W1612935534 hasConceptScore W1612935534C141315368 @default.
- W1612935534 hasConceptScore W1612935534C181199279 @default.
- W1612935534 hasConceptScore W1612935534C185592680 @default.
- W1612935534 hasConceptScore W1612935534C190062978 @default.
- W1612935534 hasConceptScore W1612935534C23265538 @default.
- W1612935534 hasConceptScore W1612935534C41625074 @default.
- W1612935534 hasConceptScore W1612935534C43617362 @default.
- W1612935534 hasConceptScore W1612935534C547475151 @default.
- W1612935534 hasConceptScore W1612935534C55493867 @default.
- W1612935534 hasConceptScore W1612935534C86803240 @default.
- W1612935534 hasConceptScore W1612935534C94412978 @default.
- W1612935534 hasLocation W16129355341 @default.
- W1612935534 hasLocation W16129355342 @default.
- W1612935534 hasOpenAccess W1612935534 @default.
- W1612935534 hasPrimaryLocation W16129355341 @default.
- W1612935534 hasRelatedWork W174238805 @default.
- W1612935534 hasRelatedWork W2022754882 @default.
- W1612935534 hasRelatedWork W2025000256 @default.
- W1612935534 hasRelatedWork W2031094128 @default.
- W1612935534 hasRelatedWork W2045308798 @default.
- W1612935534 hasRelatedWork W2056106536 @default.
- W1612935534 hasRelatedWork W2087143771 @default.
- W1612935534 hasRelatedWork W2090075052 @default.
- W1612935534 hasRelatedWork W2091977430 @default.
- W1612935534 hasRelatedWork W2258702507 @default.
- W1612935534 isParatext "false" @default.
- W1612935534 isRetracted "false" @default.
- W1612935534 magId "1612935534" @default.
- W1612935534 workType "book-chapter" @default.