Matches in SemOpenAlex for { <https://semopenalex.org/work/W161307616> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W161307616 endingPage "201" @default.
- W161307616 startingPage "195" @default.
- W161307616 abstract "This chapter discusses a few chemical reactions of N-ethylmaleimide. N-ethylmaleimide (NEM) is widely used as a reagent for blocking sulfydryl groups in proteins and peptides. The addition product of NEM with L-cysteine is easily prepared from a solution containing equimolar amounts of the reactants at pH 6. The imide ring of the addition product of NEM and cysteine is very stable to acid. Amino groups and SH groups react with NEM and hydrolysis liberates ethylamine. S-succinylcysteine is released only after reaction at sulfydryl groups. Therefore, a comparison between the amount of ethylamine and the amount of S-succinylcysteine in an acid hydrolysate of an NEM treated protein or peptide provides a quantitative measurement of the specificity of NEM toward SH groups. As ethylamine can arise also on hydrolysis of the reagent itself, NEM derivatives must be completely separated from excess reagent before hydrolysis is performed. The mechanism of the reaction of NEM with NH2-groups involves addition of the amino compound to the olefinic double bond of NEM." @default.
- W161307616 created "2016-06-24" @default.
- W161307616 creator A5009524997 @default.
- W161307616 date "1966-01-01" @default.
- W161307616 modified "2023-09-25" @default.
- W161307616 title "CHEMICAL REACTIONS OF N-ETHYLMALEIMIDE" @default.
- W161307616 doi "https://doi.org/10.1016/b978-1-4831-9851-4.50032-2" @default.
- W161307616 hasPublicationYear "1966" @default.
- W161307616 type Work @default.
- W161307616 sameAs 161307616 @default.
- W161307616 citedByCount "2" @default.
- W161307616 crossrefType "book-chapter" @default.
- W161307616 hasAuthorship W161307616A5009524997 @default.
- W161307616 hasConcept C178790620 @default.
- W161307616 hasConcept C181199279 @default.
- W161307616 hasConcept C185592680 @default.
- W161307616 hasConcept C21951064 @default.
- W161307616 hasConcept C2777646807 @default.
- W161307616 hasConcept C2778678469 @default.
- W161307616 hasConcept C2779201268 @default.
- W161307616 hasConcept C2779281246 @default.
- W161307616 hasConcept C2781338088 @default.
- W161307616 hasConcept C28800991 @default.
- W161307616 hasConcept C40875361 @default.
- W161307616 hasConcept C515207424 @default.
- W161307616 hasConcept C55493867 @default.
- W161307616 hasConcept C94412978 @default.
- W161307616 hasConceptScore W161307616C178790620 @default.
- W161307616 hasConceptScore W161307616C181199279 @default.
- W161307616 hasConceptScore W161307616C185592680 @default.
- W161307616 hasConceptScore W161307616C21951064 @default.
- W161307616 hasConceptScore W161307616C2777646807 @default.
- W161307616 hasConceptScore W161307616C2778678469 @default.
- W161307616 hasConceptScore W161307616C2779201268 @default.
- W161307616 hasConceptScore W161307616C2779281246 @default.
- W161307616 hasConceptScore W161307616C2781338088 @default.
- W161307616 hasConceptScore W161307616C28800991 @default.
- W161307616 hasConceptScore W161307616C40875361 @default.
- W161307616 hasConceptScore W161307616C515207424 @default.
- W161307616 hasConceptScore W161307616C55493867 @default.
- W161307616 hasConceptScore W161307616C94412978 @default.
- W161307616 hasLocation W1613076161 @default.
- W161307616 hasOpenAccess W161307616 @default.
- W161307616 hasPrimaryLocation W1613076161 @default.
- W161307616 hasRelatedWork W1026098380 @default.
- W161307616 hasRelatedWork W1512647055 @default.
- W161307616 hasRelatedWork W1632751105 @default.
- W161307616 hasRelatedWork W1963713347 @default.
- W161307616 hasRelatedWork W1969734584 @default.
- W161307616 hasRelatedWork W1973729871 @default.
- W161307616 hasRelatedWork W2007608609 @default.
- W161307616 hasRelatedWork W2007657130 @default.
- W161307616 hasRelatedWork W2016055123 @default.
- W161307616 hasRelatedWork W2019971818 @default.
- W161307616 hasRelatedWork W2060626118 @default.
- W161307616 hasRelatedWork W2085276762 @default.
- W161307616 hasRelatedWork W2127092618 @default.
- W161307616 hasRelatedWork W2153924083 @default.
- W161307616 hasRelatedWork W2186277040 @default.
- W161307616 hasRelatedWork W2327040934 @default.
- W161307616 hasRelatedWork W2395780953 @default.
- W161307616 hasRelatedWork W2950980709 @default.
- W161307616 hasRelatedWork W2951390260 @default.
- W161307616 hasRelatedWork W29716068 @default.
- W161307616 isParatext "false" @default.
- W161307616 isRetracted "false" @default.
- W161307616 magId "161307616" @default.
- W161307616 workType "book-chapter" @default.