Matches in SemOpenAlex for { <https://semopenalex.org/work/W1613986049> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1613986049 endingPage "724" @default.
- W1613986049 startingPage "717" @default.
- W1613986049 abstract "A newly isolated Citrobacter sp. Y19 catalyzes the CO-dependent H 2 production (biological water-gas shift reaction) by the actions of CO dehydrogenase (CODH) and hydrogenase. Y19 requires O 2 for fast growth, but its H 2 production activity is significantly inhibited by O 2 . In the present study, the effect of O 2 on the activities of CODH and hydrogenase was investigated quantitatively in both whole cells and broken cells, based on CO-dependent or methyl viologen (MV)-dependent H 2 production in addition to CO-dependent MV reduction. In crude cell extracts, CODH activity was mostly found in the soluble fraction. Inactivation of CODH and hydrogenase activities by O 2 followed the first-order decay kinetics, and the dependence of the rate constants on O 2 partial pressure could be expressed by the Michaelis-Menten equation. In whole cells, the maximum deactivation rate constants (k d,max ) of hydrogenase and CODH were quite similar: 0.07±0.03 min -1 and 0.10±0.04 min -1 , respectively. However, the first-order rate constant (k d,max /K s ) of CODH (0.25 min -1 atm -1 ) at low O 2 partial pressures was about 3-fold higher than that of the hydrogenase, since the half-saturation constant (K s ) of CODH was about half of that of hydrogenase. In broken cells, both enzymes became significantly more sensitive to O 2 compared to the unbroken cells, while k d,max /K s increased 37-fold for hydrogenase and 6.7-fold for CODH. When whole cells were incubated under anaerobic conditions after being exposed to air for 1 h, hydrogenase activity was recovered more than 90% in 2 h suggesting that the deactivation of hydrogenase by O 2 was reversible. On the contrary, CODH activity was not recovered once deactivated by O 2 , and the only way to recover the activity was to synthesize new CODH. This study indicates that O 2 sensitivity of H 2 production activity of Citrobacter sp. Y19 is an important drawback as in other H 2 -producing bacteria." @default.
- W1613986049 created "2016-06-24" @default.
- W1613986049 creator A5022918002 @default.
- W1613986049 creator A5022994723 @default.
- W1613986049 creator A5028475562 @default.
- W1613986049 creator A5052839519 @default.
- W1613986049 creator A5060644734 @default.
- W1613986049 date "2003-01-01" @default.
- W1613986049 modified "2023-09-23" @default.
- W1613986049 title "Oxygen Sensitivity of Carbon Monoxide-Dependent Hydrogen Production Activity in Citrobacter sp." @default.
- W1613986049 hasPublicationYear "2003" @default.
- W1613986049 type Work @default.
- W1613986049 sameAs 1613986049 @default.
- W1613986049 citedByCount "4" @default.
- W1613986049 crossrefType "journal-article" @default.
- W1613986049 hasAuthorship W1613986049A5022918002 @default.
- W1613986049 hasAuthorship W1613986049A5022994723 @default.
- W1613986049 hasAuthorship W1613986049A5028475562 @default.
- W1613986049 hasAuthorship W1613986049A5052839519 @default.
- W1613986049 hasAuthorship W1613986049A5060644734 @default.
- W1613986049 hasConcept C121332964 @default.
- W1613986049 hasConcept C148898269 @default.
- W1613986049 hasConcept C155647269 @default.
- W1613986049 hasConcept C161790260 @default.
- W1613986049 hasConcept C178790620 @default.
- W1613986049 hasConcept C181199279 @default.
- W1613986049 hasConcept C185592680 @default.
- W1613986049 hasConcept C2780049555 @default.
- W1613986049 hasConcept C512735826 @default.
- W1613986049 hasConcept C512968161 @default.
- W1613986049 hasConcept C55493867 @default.
- W1613986049 hasConcept C62520636 @default.
- W1613986049 hasConcept C71240020 @default.
- W1613986049 hasConcept C79115784 @default.
- W1613986049 hasConcept C93391505 @default.
- W1613986049 hasConceptScore W1613986049C121332964 @default.
- W1613986049 hasConceptScore W1613986049C148898269 @default.
- W1613986049 hasConceptScore W1613986049C155647269 @default.
- W1613986049 hasConceptScore W1613986049C161790260 @default.
- W1613986049 hasConceptScore W1613986049C178790620 @default.
- W1613986049 hasConceptScore W1613986049C181199279 @default.
- W1613986049 hasConceptScore W1613986049C185592680 @default.
- W1613986049 hasConceptScore W1613986049C2780049555 @default.
- W1613986049 hasConceptScore W1613986049C512735826 @default.
- W1613986049 hasConceptScore W1613986049C512968161 @default.
- W1613986049 hasConceptScore W1613986049C55493867 @default.
- W1613986049 hasConceptScore W1613986049C62520636 @default.
- W1613986049 hasConceptScore W1613986049C71240020 @default.
- W1613986049 hasConceptScore W1613986049C79115784 @default.
- W1613986049 hasConceptScore W1613986049C93391505 @default.
- W1613986049 hasIssue "5" @default.
- W1613986049 hasLocation W16139860491 @default.
- W1613986049 hasOpenAccess W1613986049 @default.
- W1613986049 hasPrimaryLocation W16139860491 @default.
- W1613986049 hasRelatedWork W141623723 @default.
- W1613986049 hasRelatedWork W14625545 @default.
- W1613986049 hasRelatedWork W156926467 @default.
- W1613986049 hasRelatedWork W1781980958 @default.
- W1613986049 hasRelatedWork W1970723741 @default.
- W1613986049 hasRelatedWork W2013796067 @default.
- W1613986049 hasRelatedWork W2018175038 @default.
- W1613986049 hasRelatedWork W2032611540 @default.
- W1613986049 hasRelatedWork W2035783139 @default.
- W1613986049 hasRelatedWork W2038361444 @default.
- W1613986049 hasRelatedWork W2052666483 @default.
- W1613986049 hasRelatedWork W2061150932 @default.
- W1613986049 hasRelatedWork W2084856012 @default.
- W1613986049 hasRelatedWork W2085276451 @default.
- W1613986049 hasRelatedWork W2093865478 @default.
- W1613986049 hasRelatedWork W2095262452 @default.
- W1613986049 hasRelatedWork W2103015712 @default.
- W1613986049 hasRelatedWork W2197909248 @default.
- W1613986049 hasRelatedWork W2414851483 @default.
- W1613986049 hasRelatedWork W2416953394 @default.
- W1613986049 hasVolume "13" @default.
- W1613986049 isParatext "false" @default.
- W1613986049 isRetracted "false" @default.
- W1613986049 magId "1613986049" @default.
- W1613986049 workType "article" @default.