Matches in SemOpenAlex for { <https://semopenalex.org/work/W1614067100> ?p ?o ?g. }
- W1614067100 abstract "Orc6 is a crucial component of the replication initiation machinery in eukaryotes. Its study helps us to better understand one of the most basic cell functions: DNA replication.The first step in replication initiation is the assembly and DNA binding of the origin recognition complex (ORC). Although members of ORC are highly conserved and well studied, Orc6 evolved faster than the other members of the complex, has a different structure and is less well understood than Orc1-5. The low number of studies on Orc6 in human cells and the low predictability of results obtained from model systems necessitates an in depth analysis of HsOrc6.The aim of this study is to better understand the role of Orc6 in the human cell by exploring the mechanism of Orc6-Orc1-5 interaction and Orc6-DNA binding, studying the function of Orc6 in live cells, and mapping its interaction network to find new pathways and functions of the protein.The study concludes that Orc6 interacts via its C terminus with Orc1-5 in vitro. It might also have a second pre-RC interaction domain, probably close to the nuclear localization signal. Orc6 is not required for Orc1-5-DNA binding, but is able to enhance the process in vitro. It is also able to bind DNA in the absence of Orc1-5 with high affinity.In vivo experiments present evidence on the ability of Orc6 to recruit the replication machinery to itself on DNA. Although this ability is less efficient than that of EBNA1, a viral factor, it is sufficiently strong to support autonomous replication of plasmids in human cell lines for at least four weeks. Truncated variants of the protein containing at least one of the predicted Orc1-5 interaction sites are sufficient to allow replication. This finding can be used to design autonomous-replicating plasmids for use in gene therapy.Mapping the interaction network of Orc6 leads to the conclusion that HsOrc6 is only weakly attached to pre-RC factors, and is likely to have an important role in mitosis and possibly be involved in other pathways." @default.
- W1614067100 created "2016-06-24" @default.
- W1614067100 creator A5027237152 @default.
- W1614067100 date "2014-09-19" @default.
- W1614067100 modified "2023-09-28" @default.
- W1614067100 title "The role of ORC6 in the human cell" @default.
- W1614067100 cites W143430593 @default.
- W1614067100 cites W1480720233 @default.
- W1614067100 cites W1490231232 @default.
- W1614067100 cites W1491459594 @default.
- W1614067100 cites W1542619083 @default.
- W1614067100 cites W1544733640 @default.
- W1614067100 cites W1578954095 @default.
- W1614067100 cites W1602734403 @default.
- W1614067100 cites W1605313335 @default.
- W1614067100 cites W1640120528 @default.
- W1614067100 cites W1794952483 @default.
- W1614067100 cites W1871855518 @default.
- W1614067100 cites W1886437606 @default.
- W1614067100 cites W1959134117 @default.
- W1614067100 cites W1964320907 @default.
- W1614067100 cites W1968946850 @default.
- W1614067100 cites W1970183089 @default.
- W1614067100 cites W1970515182 @default.
- W1614067100 cites W1972654719 @default.
- W1614067100 cites W1975939844 @default.
- W1614067100 cites W1976059452 @default.
- W1614067100 cites W1978617734 @default.
- W1614067100 cites W1979282401 @default.
- W1614067100 cites W1982543117 @default.
- W1614067100 cites W1983356442 @default.
- W1614067100 cites W1985481802 @default.
- W1614067100 cites W1987945388 @default.
- W1614067100 cites W1990953761 @default.
- W1614067100 cites W1993417549 @default.
- W1614067100 cites W1994945217 @default.
- W1614067100 cites W1995425986 @default.
- W1614067100 cites W1995434980 @default.
- W1614067100 cites W2001163515 @default.
- W1614067100 cites W2001222878 @default.
- W1614067100 cites W2002326161 @default.
- W1614067100 cites W2003945173 @default.
- W1614067100 cites W2004115073 @default.
- W1614067100 cites W2005705565 @default.
- W1614067100 cites W2006323365 @default.
- W1614067100 cites W2007214324 @default.
- W1614067100 cites W2008925499 @default.
- W1614067100 cites W2011462883 @default.
- W1614067100 cites W2014024536 @default.
- W1614067100 cites W2014431326 @default.
- W1614067100 cites W2014717496 @default.
- W1614067100 cites W2015155762 @default.
- W1614067100 cites W2015210486 @default.
- W1614067100 cites W2017472700 @default.
- W1614067100 cites W2020058603 @default.
- W1614067100 cites W2026613581 @default.
- W1614067100 cites W2026821511 @default.
- W1614067100 cites W2029423003 @default.
- W1614067100 cites W2030665161 @default.
- W1614067100 cites W2030974202 @default.
- W1614067100 cites W2031954637 @default.
- W1614067100 cites W2033722975 @default.
- W1614067100 cites W2034418355 @default.
- W1614067100 cites W2034458429 @default.
- W1614067100 cites W2035313782 @default.
- W1614067100 cites W2035982764 @default.
- W1614067100 cites W2036008069 @default.
- W1614067100 cites W2037610353 @default.
- W1614067100 cites W2038174431 @default.
- W1614067100 cites W2038427905 @default.
- W1614067100 cites W2039577300 @default.
- W1614067100 cites W2042154380 @default.
- W1614067100 cites W2044279758 @default.
- W1614067100 cites W2045327902 @default.
- W1614067100 cites W2045513157 @default.
- W1614067100 cites W2047600396 @default.
- W1614067100 cites W2049504217 @default.
- W1614067100 cites W2050796617 @default.
- W1614067100 cites W2052489882 @default.
- W1614067100 cites W2052992525 @default.
- W1614067100 cites W2053437887 @default.
- W1614067100 cites W2054814766 @default.
- W1614067100 cites W2055524182 @default.
- W1614067100 cites W2055792343 @default.
- W1614067100 cites W2056590571 @default.
- W1614067100 cites W2059594509 @default.
- W1614067100 cites W2061449183 @default.
- W1614067100 cites W2061481911 @default.
- W1614067100 cites W2062012662 @default.
- W1614067100 cites W2063259131 @default.
- W1614067100 cites W206344101 @default.
- W1614067100 cites W2063543381 @default.
- W1614067100 cites W2065971145 @default.
- W1614067100 cites W2067666713 @default.
- W1614067100 cites W2067797937 @default.
- W1614067100 cites W2070050544 @default.
- W1614067100 cites W2070358989 @default.
- W1614067100 cites W2070435451 @default.
- W1614067100 cites W2070511522 @default.
- W1614067100 cites W2072585523 @default.