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- W161628279 abstract "Quantitative analysis of RNA is an important aspect of gene expression studies and central to the understanding of the mechanisms that regulate gene activity. Northern gels, dot or slot blots, are currently used for analyzing RNA levels, however, Northern analysis requires large quantities of RNA and is not very sensitive for quantitative use in detecting low abundance mRNA. S1 nuclease assays, RNase A protection, and in situ hybridization methods are more sensitive and quantitative, however, these methods are time consuming, can be technically difficult, and are not useful for a simultaneous processing of a large number of samples. Although these methods are more sensitive than Northern analysis, for many practical purposes, i.e., analyzing low copy number of transcripts or small amount of cells, the very low levels of mRNA limit their widespread use." @default.
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- W161628279 date "1994-01-01" @default.
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- W161628279 title "RT-PCR and mRNA Quantitation" @default.
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