Matches in SemOpenAlex for { <https://semopenalex.org/work/W161728519> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W161728519 endingPage "52" @default.
- W161728519 startingPage "S41" @default.
- W161728519 abstract "SIVsm chronically infected cultures were obtained after infection of CEMX174 cells with either SIVsmH3 or SIVsmE660. These phenotypically CD4 cells, formed syncytia but only when cocultivated with CD4+ cells. Single cell clones were derived from these cultures and examined for the production of virus-specific proteins. The majority of the clones expressed SIV p27 antigen and low levels of virus reverse transcriptase activity. Western blot analysis, performed with either monoclonal or polyclonal sera, showed that a chronically infected clone (B7) produced particles which contained envelope (gp135 and gp43), gag precursors and gag proteins (p27, p16 and p8). However, these particles (SIVsmB7) lacked detectable levels of vpx and of integrase, and contained several fusion proteins which expressed viral protease antigens. This defective virus failed to infect established CD4+ cell lines, as well as primary cultures of macrophages and of peripheral blood lymphocytes, obtained both from humans and from rhesus macaques. Lack of infection correlated with lack of viral DNA detection by PCR amplification of genomic DNA extracted from these cell cultures. In addition, SIVsmB7 virus lacked infectivity in vivo. Rhesus macaques inoculated with high concentrations of SIVsmB7 showed no viremia and their PBMC were PCR negative. Thus, B7 cells produced stable, non-infectious virus mutants, which contained env and gag proteins, but lacked detectable amounts of vpx and of enzymes required for virus replication. Due to the high constitutive expression of this virus-like particle, we are now testing this preparation as a vaccine." @default.
- W161728519 created "2016-06-24" @default.
- W161728519 creator A5033754409 @default.
- W161728519 creator A5041848775 @default.
- W161728519 date "1995-01-01" @default.
- W161728519 modified "2023-10-03" @default.
- W161728519 title "Properties of virus-like particles produced by SIV-chronically infected human cell clones." @default.
- W161728519 cites W1579711827 @default.
- W161728519 cites W1824849326 @default.
- W161728519 cites W1970407061 @default.
- W161728519 cites W1981864966 @default.
- W161728519 cites W2002292720 @default.
- W161728519 cites W2060142383 @default.
- W161728519 cites W2061508577 @default.
- W161728519 cites W2062019922 @default.
- W161728519 cites W2089471930 @default.
- W161728519 cites W2101108802 @default.
- W161728519 cites W2135539943 @default.
- W161728519 cites W2396557594 @default.
- W161728519 cites W2402144987 @default.
- W161728519 cites W2409508182 @default.
- W161728519 cites W2409769001 @default.
- W161728519 cites W2412754347 @default.
- W161728519 cites W2415250205 @default.
- W161728519 cites W2462228587 @default.
- W161728519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8574147" @default.
- W161728519 hasPublicationYear "1995" @default.
- W161728519 type Work @default.
- W161728519 sameAs 161728519 @default.
- W161728519 citedByCount "7" @default.
- W161728519 crossrefType "journal-article" @default.
- W161728519 hasAuthorship W161728519A5033754409 @default.
- W161728519 hasAuthorship W161728519A5041848775 @default.
- W161728519 hasConcept C104317684 @default.
- W161728519 hasConcept C106358424 @default.
- W161728519 hasConcept C137061746 @default.
- W161728519 hasConcept C140704245 @default.
- W161728519 hasConcept C147483822 @default.
- W161728519 hasConcept C153911025 @default.
- W161728519 hasConcept C156719811 @default.
- W161728519 hasConcept C159047783 @default.
- W161728519 hasConcept C202751555 @default.
- W161728519 hasConcept C2522874641 @default.
- W161728519 hasConcept C2776185481 @default.
- W161728519 hasConcept C49105822 @default.
- W161728519 hasConcept C54355233 @default.
- W161728519 hasConcept C552990157 @default.
- W161728519 hasConcept C55493867 @default.
- W161728519 hasConcept C81089528 @default.
- W161728519 hasConcept C81885089 @default.
- W161728519 hasConcept C86803240 @default.
- W161728519 hasConceptScore W161728519C104317684 @default.
- W161728519 hasConceptScore W161728519C106358424 @default.
- W161728519 hasConceptScore W161728519C137061746 @default.
- W161728519 hasConceptScore W161728519C140704245 @default.
- W161728519 hasConceptScore W161728519C147483822 @default.
- W161728519 hasConceptScore W161728519C153911025 @default.
- W161728519 hasConceptScore W161728519C156719811 @default.
- W161728519 hasConceptScore W161728519C159047783 @default.
- W161728519 hasConceptScore W161728519C202751555 @default.
- W161728519 hasConceptScore W161728519C2522874641 @default.
- W161728519 hasConceptScore W161728519C2776185481 @default.
- W161728519 hasConceptScore W161728519C49105822 @default.
- W161728519 hasConceptScore W161728519C54355233 @default.
- W161728519 hasConceptScore W161728519C552990157 @default.
- W161728519 hasConceptScore W161728519C55493867 @default.
- W161728519 hasConceptScore W161728519C81089528 @default.
- W161728519 hasConceptScore W161728519C81885089 @default.
- W161728519 hasConceptScore W161728519C86803240 @default.
- W161728519 hasLocation W1617285191 @default.
- W161728519 hasOpenAccess W161728519 @default.
- W161728519 hasPrimaryLocation W1617285191 @default.
- W161728519 hasRelatedWork W161728519 @default.
- W161728519 hasRelatedWork W1979532458 @default.
- W161728519 hasRelatedWork W2065190721 @default.
- W161728519 hasRelatedWork W2078027579 @default.
- W161728519 hasRelatedWork W2091270288 @default.
- W161728519 hasRelatedWork W2152630682 @default.
- W161728519 hasRelatedWork W2155708590 @default.
- W161728519 hasRelatedWork W2325872233 @default.
- W161728519 hasRelatedWork W2422228764 @default.
- W161728519 hasRelatedWork W572320671 @default.
- W161728519 hasVolume "41 Suppl 1" @default.
- W161728519 isParatext "false" @default.
- W161728519 isRetracted "false" @default.
- W161728519 magId "161728519" @default.
- W161728519 workType "article" @default.