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• W1617602615 abstract "We describe a novel two-photon (2P) laser scanning microscopy (2PLSM) protocol that provides ratiometric transmural measurements of membrane voltage (Vm ) via Di-4-ANEPPS in intact mouse, rat and rabbit hearts with subcellular resolution. The same cells were then imaged with Fura-2/AM for intracellular Ca(2+) recordings. Action potentials (APs) were accurately characterized by 2PLSM vs. microelectrodes, albeit fast events (<1 ms) were sub-optimally acquired by 2PLSM due to limited sampling frequencies (2.6 kHz). The slower Ca(2+) transient (CaT) time course (>1ms) could be accurately described by 2PLSM. In conclusion, Vm - and Ca(2+) -sensitive dyes can be 2P excited within the cardiac muscle wall to provide AP and Ca(2+) signals to ∼400 µm." @default.
• W1617602615 created "2016-06-24" @default.
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• W1617602615 date "2013-10-02" @default.
• W1617602615 modified "2023-09-24" @default.
• W1617602615 title "2-photon excitation fluorescence microscopy enables deeper high-resolution imaging of voltage and Ca²⁺in intact mice, rat, and rabbit hearts" @default.
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• W1617602615 doi "https://doi.org/10.1002/jbio.201300109" @default.
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