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• W161819005 startingPage "329" @default.
• W161819005 abstract "In the early 1990s, an obstetrician colleague and I came to the conclusion that looking for sperm-specific RNA by RT-PCR in the ejaculate of recently vasectomized men might be a more sensitive (and convenient) way of determining whether the operation had been a success. A pilot study was conducted (never published), which showed clearly that molecular methods could indeed be a more efficient and accurate alternative for detecting residual spermatozoa in a sterilized ejaculate than manually scanning through many semen samples under the microscope. Shortly thereafter, the first reports appeared in the literature documenting the presence of mRNA in human spermatozoa by RT-PCR. Sperm RNA was an unexpected discovery for many andrological biologists because it had been generally assumed long before that RNA was lost or degraded during the extensive cellular remodeling that spermatids undergo during spermiogenesis, when nuclear condensation is accompanied by the loss of most of the cytoplasm (and cytoplasmic RNA) and the termination of protein synthesis. Hence, the detection of fully processed intact mRNA in mature ejaculate spermatozoa was treated with some scepticism. Some critics considered that sperm RNA was an artifact caused by immature round or somatic cell contamination of semen samples. Others felt that sperm RNA was (at best) a minor residual component of the mature gamete that served no useful purpose. Both criticisms were subsequently challenged by compelling experiments showing that sperm RNA is unequivocally spermatozoal in origin and that regardless of its passive diagnostic potential in a fertility context, sperm RNA probably performs active functions both within the cell itself and perhaps more intriguingly, once it gains access to the ooplasm. The focus of this chapter is on the discovery of sperm RNA and the historical and contemporary research that has provided some important clues to its function. It will include discussion of its utility in a diagnostic context (for male infertility) and of recent interesting work suggesting that sperm RNA is part of and evidence for a wider mechanism for reshaping the genome that augments its more obvious epigenetic potential as detailed elsewhere in this volume." @default.
• W161819005 created "2016-06-24" @default.
• W161819005 creator A5032535992 @default.
• W161819005 date "2010-10-14" @default.
• W161819005 modified "2023-09-27" @default.
• W161819005 title "Sperm RNA: Reading the Hidden Message" @default.
• W161819005 cites W1491910236 @default.
• W161819005 cites W1571774167 @default.
• W161819005 cites W17703454 @default.
• W161819005 cites W1859101681 @default.
• W161819005 cites W1912418335 @default.
• W161819005 cites W1964409511 @default.
• W161819005 cites W1966285167 @default.
• W161819005 cites W1966579785 @default.
• W161819005 cites W1966972617 @default.
• W161819005 cites W1971074826 @default.
• W161819005 cites W1972914977 @default.
• W161819005 cites W1973842956 @default.
• W161819005 cites W1973847022 @default.
• W161819005 cites W1974235411 @default.
• W161819005 cites W1976822936 @default.
• W161819005 cites W1979238526 @default.
• W161819005 cites W1979635135 @default.
• W161819005 cites W1980577785 @default.
• W161819005 cites W1980972550 @default.
• W161819005 cites W1981003844 @default.
• W161819005 cites W1984725572 @default.
• W161819005 cites W1987814926 @default.
• W161819005 cites W1991130456 @default.
• W161819005 cites W1991910205 @default.
• W161819005 cites W1992466559 @default.
• W161819005 cites W1992770121 @default.
• W161819005 cites W1993061318 @default.
• W161819005 cites W1993287793 @default.
• W161819005 cites W1993290079 @default.
• W161819005 cites W1997347275 @default.
• W161819005 cites W2000261266 @default.
• W161819005 cites W2007178024 @default.
• W161819005 cites W2008019350 @default.
• W161819005 cites W2008817503 @default.
• W161819005 cites W2010868220 @default.
• W161819005 cites W2010994533 @default.
• W161819005 cites W2011213056 @default.
• W161819005 cites W2015120218 @default.
• W161819005 cites W2018221845 @default.
• W161819005 cites W2020185658 @default.
• W161819005 cites W2020582212 @default.
• W161819005 cites W2023374233 @default.
• W161819005 cites W2024785223 @default.
• W161819005 cites W2025279977 @default.
• W161819005 cites W2025288035 @default.
• W161819005 cites W2026530186 @default.
• W161819005 cites W2028079404 @default.
• W161819005 cites W2028961360 @default.
• W161819005 cites W2029339391 @default.
• W161819005 cites W2029694986 @default.
• W161819005 cites W2032878693 @default.
• W161819005 cites W2034548201 @default.
• W161819005 cites W2035074912 @default.
• W161819005 cites W2035627755 @default.
• W161819005 cites W2035930185 @default.
• W161819005 cites W2038127200 @default.
• W161819005 cites W2038231313 @default.
• W161819005 cites W2039968377 @default.
• W161819005 cites W2041482906 @default.
• W161819005 cites W2043686232 @default.
• W161819005 cites W2044922726 @default.
• W161819005 cites W2045220576 @default.
• W161819005 cites W2049223323 @default.
• W161819005 cites W2049353645 @default.
• W161819005 cites W2052764329 @default.
• W161819005 cites W2055821541 @default.
• W161819005 cites W2057868893 @default.
• W161819005 cites W2059053699 @default.
• W161819005 cites W2061223196 @default.
• W161819005 cites W2061569670 @default.
• W161819005 cites W2063955247 @default.
• W161819005 cites W2069732337 @default.
• W161819005 cites W2070319253 @default.
• W161819005 cites W2070807615 @default.
• W161819005 cites W2077156813 @default.
• W161819005 cites W2077253418 @default.
• W161819005 cites W2078490448 @default.
• W161819005 cites W2078561308 @default.
• W161819005 cites W2082061523 @default.
• W161819005 cites W2083210315 @default.
• W161819005 cites W2088029632 @default.
• W161819005 cites W2090723511 @default.
• W161819005 cites W2091883582 @default.
• W161819005 cites W2096393864 @default.
• W161819005 cites W2096927360 @default.
• W161819005 cites W2100200289 @default.
• W161819005 cites W2100269956 @default.
• W161819005 cites W2101092732 @default.
• W161819005 cites W2103012554 @default.
• W161819005 cites W2103014655 @default.
• W161819005 cites W2103041850 @default.
• W161819005 cites W2103336074 @default.

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