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- W1619003858 abstract "This chapter describes two general techniques for preparing fragments of the large ribosomal RNAs. A hybridization selection scheme cuts the desired fragment out of purified rRNA. This method is inconvenient for preparing large amounts of RNA, but preserves modified bases in the sequence. The second method is in vitro transcription of the RNA from a DNA template. This method is suitable for preparing large quantifies of RNA, and can also be used to prepare a series of sequence variants. RNA fragments, prepared for studies of the central domain of the 16S rRNA, will be described. Cloning vectors containing promoters for SP6, T3, and T7 phage promoters, as well as the phage RNA polymerases, are commercially available. These systems were designed for the preparation of single-stranded hybridization probes. Some of the phage polymerases are easy to purify in large quantities. Since the phage T7 RNA polymerase has been expressed at very high levels in E. coil it is currently the best choice when RNA is to be made for physical studies, and this article will deal only with it." @default.
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- W1619003858 date "1988-01-01" @default.
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- W1619003858 title "[14] Preparation of specific ribosomal RNA fragments" @default.
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- W1619003858 doi "https://doi.org/10.1016/s0076-6879(88)64045-6" @default.
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