Matches in SemOpenAlex for { <https://semopenalex.org/work/W1620174037> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1620174037 endingPage "403" @default.
- W1620174037 startingPage "395" @default.
- W1620174037 abstract "As part of a continuing study of the induction of alkaloid biosynthesis, we report the isolation to homogeneity and characterization of S-adenosyl-L-methionine:tetrahydroberberine-cis-N-mehtyltransferase from suspension cultures of Sanguinaria canadensis that were induced to produce alkaloids by hormone depletion. This enzyme catalyzes the stereospecific transfer of a methyl group from S-adenosyl-L-methionine to the tertiary nitrogen of the protoberberine alkaloid tetrahydroberberine (canadine). The enzyme was purified 315-fold by ammonium sulfate precipitation, gel permeation chromatography, affinity dye chromatography, and both diethylaminoethyl and Mono-Q ion-exchange chromatography. The enzyme was further purified to an optimum specific activity of 225 nkat/mg of protein (3500-fold) and electrophoretic homogeneity by native polyacrylamide gel electrophoresis (PAGE). In contrast to previous reports with partially purified enzyme, the isolated protein was found to have a pH optimum of 7.0, a temperature optimum of 25 to 30[deg]C, and an isoelectric point of 5.1. Furthermore, the molecular weight of the homogeneous protein was found to be 39,000 by sodium dodecyl sulfate-PAGE. The homogeneous enzyme preferred tetrahydroberberine over all other substrates tested, showing an apparent Km of 2.1 [mu]M, but also showed partial activity with tetrahydrojatrorrhizine and tetrahydropalmatrubine." @default.
- W1620174037 created "2016-06-24" @default.
- W1620174037 creator A5018739306 @default.
- W1620174037 creator A5067064525 @default.
- W1620174037 date "1994-05-01" @default.
- W1620174037 modified "2023-09-23" @default.
- W1620174037 title "Isolation and Characterization of S-Adenosyl-L-Methionine:Tetrahydroberberine-cis-N-Methyltransferase from Suspension Cultures of Sanguinaria canadensis L" @default.
- W1620174037 cites W2016335922 @default.
- W1620174037 cites W2018289835 @default.
- W1620174037 cites W2036104262 @default.
- W1620174037 cites W2048758356 @default.
- W1620174037 cites W2072478286 @default.
- W1620174037 cites W2085547973 @default.
- W1620174037 cites W2100837269 @default.
- W1620174037 cites W2128635872 @default.
- W1620174037 doi "https://doi.org/10.1104/pp.105.1.395" @default.
- W1620174037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/159368" @default.
- W1620174037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12232209" @default.
- W1620174037 hasPublicationYear "1994" @default.
- W1620174037 type Work @default.
- W1620174037 sameAs 1620174037 @default.
- W1620174037 citedByCount "18" @default.
- W1620174037 countsByYear W16201740372014 @default.
- W1620174037 countsByYear W16201740372016 @default.
- W1620174037 countsByYear W16201740372018 @default.
- W1620174037 countsByYear W16201740372020 @default.
- W1620174037 crossrefType "journal-article" @default.
- W1620174037 hasAuthorship W1620174037A5018739306 @default.
- W1620174037 hasAuthorship W1620174037A5067064525 @default.
- W1620174037 hasBestOaLocation W16201740371 @default.
- W1620174037 hasConcept C101660505 @default.
- W1620174037 hasConcept C163994747 @default.
- W1620174037 hasConcept C179247698 @default.
- W1620174037 hasConcept C181199279 @default.
- W1620174037 hasConcept C185592680 @default.
- W1620174037 hasConcept C193363816 @default.
- W1620174037 hasConcept C2776428949 @default.
- W1620174037 hasConcept C2777667214 @default.
- W1620174037 hasConcept C2778046504 @default.
- W1620174037 hasConcept C2780912031 @default.
- W1620174037 hasConcept C43617362 @default.
- W1620174037 hasConcept C515207424 @default.
- W1620174037 hasConcept C51724486 @default.
- W1620174037 hasConcept C55493867 @default.
- W1620174037 hasConcept C71240020 @default.
- W1620174037 hasConcept C96294017 @default.
- W1620174037 hasConceptScore W1620174037C101660505 @default.
- W1620174037 hasConceptScore W1620174037C163994747 @default.
- W1620174037 hasConceptScore W1620174037C179247698 @default.
- W1620174037 hasConceptScore W1620174037C181199279 @default.
- W1620174037 hasConceptScore W1620174037C185592680 @default.
- W1620174037 hasConceptScore W1620174037C193363816 @default.
- W1620174037 hasConceptScore W1620174037C2776428949 @default.
- W1620174037 hasConceptScore W1620174037C2777667214 @default.
- W1620174037 hasConceptScore W1620174037C2778046504 @default.
- W1620174037 hasConceptScore W1620174037C2780912031 @default.
- W1620174037 hasConceptScore W1620174037C43617362 @default.
- W1620174037 hasConceptScore W1620174037C515207424 @default.
- W1620174037 hasConceptScore W1620174037C51724486 @default.
- W1620174037 hasConceptScore W1620174037C55493867 @default.
- W1620174037 hasConceptScore W1620174037C71240020 @default.
- W1620174037 hasConceptScore W1620174037C96294017 @default.
- W1620174037 hasIssue "1" @default.
- W1620174037 hasLocation W16201740371 @default.
- W1620174037 hasLocation W16201740372 @default.
- W1620174037 hasLocation W16201740373 @default.
- W1620174037 hasOpenAccess W1620174037 @default.
- W1620174037 hasPrimaryLocation W16201740371 @default.
- W1620174037 hasRelatedWork W1649084527 @default.
- W1620174037 hasRelatedWork W2002722228 @default.
- W1620174037 hasRelatedWork W2022968415 @default.
- W1620174037 hasRelatedWork W2026191303 @default.
- W1620174037 hasRelatedWork W2037142087 @default.
- W1620174037 hasRelatedWork W2074621646 @default.
- W1620174037 hasRelatedWork W2138791828 @default.
- W1620174037 hasRelatedWork W2387051580 @default.
- W1620174037 hasRelatedWork W2411010676 @default.
- W1620174037 hasRelatedWork W2583986277 @default.
- W1620174037 hasVolume "105" @default.
- W1620174037 isParatext "false" @default.
- W1620174037 isRetracted "false" @default.
- W1620174037 magId "1620174037" @default.
- W1620174037 workType "article" @default.