Matches in SemOpenAlex for { <https://semopenalex.org/work/W1621327294> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1621327294 endingPage "55" @default.
- W1621327294 startingPage "49" @default.
- W1621327294 abstract "Abstract Ecdysone 20-monooxygenase, the enzyme system that hydroxylates ecdysone to 20-hydroxyecdysone, was characterized in wandering stage larvae of Drosophila melanogaster and blood fed adult female Aedes aegypti using an in vitro radioassay in conjunction with analytical thin layer chromatography. Preliminary data suggested that ecdysone 20-monooxygenase activity is present in body wall/fat body complexes, gut/Malpighian tubule complexes and ovaries of Aedes and that this enzyme activity varies in a tissue-specific fashion during the gonadotropic cycle. The 20-monooxygenase in both Drosophila and Aedes was most active in sodium phosphate buffer, pH 7.5, and exhibited a temperature optimum at 35°C. Differential centrifugation has revealed that ecdysone 20-monooxygenase activity is associated with both the mitochondrial and microsomal fractions of Aedes and Drosophila and this has been confirmed in Drosophila by sucrose step gradient centrifugation, electron microscopy and organellemarker enzyme analysis. Substrate kinetics experiments have shown that both the Aedes and Drosophila monooxygenase systems have low apparent Kms for ecdysone in the range of 0.6–3 × 10 −7 M . Both Drosophila and Aedes monooxygenase systems were inhibited by 20-hydroxyecdysone. The cytochrome P-450 dependency of the Drosophila monooxygenase systems was established by cofactor requirements, inhibitor studies and photochemical action spectra determinations. The cytochrome P-450 dependency of the Aedes monooxygenase systems was suggested by their cofactor requirements and inhibitor studies." @default.
- W1621327294 created "2016-06-24" @default.
- W1621327294 creator A5026804374 @default.
- W1621327294 creator A5061938138 @default.
- W1621327294 date "1986-01-01" @default.
- W1621327294 modified "2023-09-24" @default.
- W1621327294 title "ECDYSONE 20-MONOOXYGENASE SYSTEMS IN A LARVAL AND AN ADULT DIPTERAN: AN OVERVIEW OF THEIR BIOCHEMISTRY, PHYSIOLOGY AND PHARMACOLOGY" @default.
- W1621327294 cites W1026647789 @default.
- W1621327294 cites W1482186717 @default.
- W1621327294 cites W192784235 @default.
- W1621327294 cites W1973597761 @default.
- W1621327294 cites W1974584213 @default.
- W1621327294 cites W1979889134 @default.
- W1621327294 cites W1988624592 @default.
- W1621327294 cites W2011759977 @default.
- W1621327294 cites W2015707287 @default.
- W1621327294 cites W2024831301 @default.
- W1621327294 cites W2026041854 @default.
- W1621327294 cites W2031262510 @default.
- W1621327294 cites W2038229929 @default.
- W1621327294 cites W2046881348 @default.
- W1621327294 cites W2051843033 @default.
- W1621327294 cites W2057646889 @default.
- W1621327294 cites W2078806857 @default.
- W1621327294 cites W2086522423 @default.
- W1621327294 cites W2088379748 @default.
- W1621327294 cites W2090253724 @default.
- W1621327294 cites W2095357678 @default.
- W1621327294 cites W2150157881 @default.
- W1621327294 cites W230114769 @default.
- W1621327294 cites W2323906957 @default.
- W1621327294 cites W2415473005 @default.
- W1621327294 cites W2978776366 @default.
- W1621327294 cites W304963244 @default.
- W1621327294 hasPublicationYear "1986" @default.
- W1621327294 type Work @default.
- W1621327294 sameAs 1621327294 @default.
- W1621327294 citedByCount "2" @default.
- W1621327294 crossrefType "journal-article" @default.
- W1621327294 hasAuthorship W1621327294A5026804374 @default.
- W1621327294 hasAuthorship W1621327294A5061938138 @default.
- W1621327294 hasConcept C104317684 @default.
- W1621327294 hasConcept C13002179 @default.
- W1621327294 hasConcept C150149183 @default.
- W1621327294 hasConcept C173758957 @default.
- W1621327294 hasConcept C181199279 @default.
- W1621327294 hasConcept C22145727 @default.
- W1621327294 hasConcept C2777180181 @default.
- W1621327294 hasConcept C2777775583 @default.
- W1621327294 hasConcept C2780104201 @default.
- W1621327294 hasConcept C526171541 @default.
- W1621327294 hasConcept C55493867 @default.
- W1621327294 hasConcept C59822182 @default.
- W1621327294 hasConcept C71315377 @default.
- W1621327294 hasConcept C86803240 @default.
- W1621327294 hasConceptScore W1621327294C104317684 @default.
- W1621327294 hasConceptScore W1621327294C13002179 @default.
- W1621327294 hasConceptScore W1621327294C150149183 @default.
- W1621327294 hasConceptScore W1621327294C173758957 @default.
- W1621327294 hasConceptScore W1621327294C181199279 @default.
- W1621327294 hasConceptScore W1621327294C22145727 @default.
- W1621327294 hasConceptScore W1621327294C2777180181 @default.
- W1621327294 hasConceptScore W1621327294C2777775583 @default.
- W1621327294 hasConceptScore W1621327294C2780104201 @default.
- W1621327294 hasConceptScore W1621327294C526171541 @default.
- W1621327294 hasConceptScore W1621327294C55493867 @default.
- W1621327294 hasConceptScore W1621327294C59822182 @default.
- W1621327294 hasConceptScore W1621327294C71315377 @default.
- W1621327294 hasConceptScore W1621327294C86803240 @default.
- W1621327294 hasIssue "1" @default.
- W1621327294 hasLocation W16213272941 @default.
- W1621327294 hasOpenAccess W1621327294 @default.
- W1621327294 hasPrimaryLocation W16213272941 @default.
- W1621327294 hasRelatedWork W1482186717 @default.
- W1621327294 hasRelatedWork W173647254 @default.
- W1621327294 hasRelatedWork W1973597761 @default.
- W1621327294 hasRelatedWork W1974584213 @default.
- W1621327294 hasRelatedWork W1988624592 @default.
- W1621327294 hasRelatedWork W2003508487 @default.
- W1621327294 hasRelatedWork W2011759977 @default.
- W1621327294 hasRelatedWork W2015707287 @default.
- W1621327294 hasRelatedWork W2018997046 @default.
- W1621327294 hasRelatedWork W2027498096 @default.
- W1621327294 hasRelatedWork W2033002048 @default.
- W1621327294 hasRelatedWork W2056676876 @default.
- W1621327294 hasRelatedWork W2064083021 @default.
- W1621327294 hasRelatedWork W2090253724 @default.
- W1621327294 hasRelatedWork W2095357678 @default.
- W1621327294 hasRelatedWork W230114769 @default.
- W1621327294 hasRelatedWork W2415473005 @default.
- W1621327294 hasRelatedWork W2896577084 @default.
- W1621327294 hasRelatedWork W2978776366 @default.
- W1621327294 hasRelatedWork W591808928 @default.
- W1621327294 hasVolume "16" @default.
- W1621327294 isParatext "false" @default.
- W1621327294 isRetracted "false" @default.
- W1621327294 magId "1621327294" @default.
- W1621327294 workType "article" @default.