FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1623367170> ?p ?o ?g. }

• W1623367170 endingPage "693" @default.
• W1623367170 startingPage "683" @default.
• W1623367170 abstract "Current approaches to cartilage tissue engineering require a large number of chondrocytes. Although chondrocyte numbers can be expanded in monolayer culture, the cells dedifferentiate and unless they can be redifferentiated are not optimal to use for cartilage repair. We took advantage of the differential effect of culture conditions on the ability of passaged and primary chondrocytes to form cartilage tissue to dissect out the extracellular matrix (ECM) molecules produced and accumulated in the early stages of passaged cell cartilage tissue formation as we hypothesized that passaged bovine cells that form cartilage accumulate a pericellular matrix that differs from cells that do not form cartilage. Twice passaged bovine chondrocytes (P2) (cartilage forming), or as a control primary chondrocytes (P0) (which do not generate cartilage), were cultured on three-dimensional membrane inserts in serum-free media. P2 redifferentiation was occurring during the first 8 days as indicated by increased expression of the chondrogenic genes Sox9, collagen type II, aggrecan, and COMP, suggesting that this is an appropriate time period to examine the ECM. Mass spectrometry showed that the P2 secretome (molecules released into the media) at 1 week had higher levels of collagen types I, III, and XII, and versican while type II collagen and COMP were found at higher levels in the P0 secretome. There was increased collagen synthesis and retention by P2 cells compared to P0 cells as early as 3 days of culture. Confocal microscopy showed that types XII, III, and II collagen, aggrecan, versican, and decorin were present in the ECM of P2 cells. In contrast, collagen types I, II, and III, aggrecan, and decorin were present in the ECM of P0 cells. As primary chondrocytes grown in serum-containing media, a condition that allows for the generation of cartilage tissue in vitro, also accumulate versican and collagen XII, this study suggests that these molecules may be necessary to provide a microenvironment that supports hyaline cartilage formation. Further study is required to determine if these molecules are also accumulated by passaged human chondrocytes and their role in promoting hyaline cartilage formation." @default.
• W1623367170 created "2016-06-24" @default.
• W1623367170 creator A5001467466 @default.
• W1623367170 creator A5021491230 @default.
• W1623367170 creator A5052949728 @default.
• W1623367170 creator A5060444850 @default.
• W1623367170 creator A5081883425 @default.
• W1623367170 creator A5084226800 @default.
• W1623367170 creator A5086511870 @default.
• W1623367170 date "2015-02-01" @default.
• W1623367170 modified "2023-09-27" @default.
• W1623367170 title "Collagen Type XII and Versican Are Present in the Early Stages of Cartilage Tissue Formation by Both Redifferentating Passaged and Primary Chondrocytes" @default.
• W1623367170 cites W1496679328 @default.
• W1623367170 cites W1525977186 @default.
• W1623367170 cites W170206043 @default.
• W1623367170 cites W1814014218 @default.
• W1623367170 cites W1959440999 @default.
• W1623367170 cites W1967683284 @default.
• W1623367170 cites W1969219199 @default.
• W1623367170 cites W1969729923 @default.
• W1623367170 cites W1973198536 @default.
• W1623367170 cites W1988930710 @default.
• W1623367170 cites W1990461498 @default.
• W1623367170 cites W1998112612 @default.
• W1623367170 cites W2002934536 @default.
• W1623367170 cites W2006554928 @default.
• W1623367170 cites W2017940237 @default.
• W1623367170 cites W2028690282 @default.
• W1623367170 cites W2029164592 @default.
• W1623367170 cites W2044666305 @default.
• W1623367170 cites W2049442243 @default.
• W1623367170 cites W2050078783 @default.
• W1623367170 cites W2052890518 @default.
• W1623367170 cites W2056523181 @default.
• W1623367170 cites W2057991588 @default.
• W1623367170 cites W2066221839 @default.
• W1623367170 cites W2066346262 @default.
• W1623367170 cites W2067034977 @default.
• W1623367170 cites W2067973562 @default.
• W1623367170 cites W2070396748 @default.
• W1623367170 cites W2078538657 @default.
• W1623367170 cites W2080242496 @default.
• W1623367170 cites W2084307204 @default.
• W1623367170 cites W2086495248 @default.
• W1623367170 cites W2089567262 @default.
• W1623367170 cites W2093993567 @default.
• W1623367170 cites W2142621773 @default.
• W1623367170 cites W2147471389 @default.
• W1623367170 cites W2152392358 @default.
• W1623367170 cites W2157827546 @default.
• W1623367170 cites W2159601540 @default.
• W1623367170 cites W2161887455 @default.
• W1623367170 cites W2166605948 @default.
• W1623367170 cites W2169421409 @default.
• W1623367170 cites W2169725609 @default.
• W1623367170 cites W2315472330 @default.
• W1623367170 cites W2320441563 @default.
• W1623367170 cites W4250595858 @default.
• W1623367170 cites W7338016 @default.
• W1623367170 doi "https://doi.org/10.1089/ten.tea.2014.0103" @default.
• W1623367170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25315796" @default.
• W1623367170 hasPublicationYear "2015" @default.
• W1623367170 type Work @default.
• W1623367170 sameAs 1623367170 @default.
• W1623367170 citedByCount "28" @default.
• W1623367170 countsByYear W16233671702015 @default.
• W1623367170 countsByYear W16233671702016 @default.
• W1623367170 countsByYear W16233671702017 @default.
• W1623367170 countsByYear W16233671702018 @default.
• W1623367170 countsByYear W16233671702019 @default.
• W1623367170 countsByYear W16233671702020 @default.
• W1623367170 countsByYear W16233671702021 @default.
• W1623367170 countsByYear W16233671702022 @default.
• W1623367170 crossrefType "journal-article" @default.
• W1623367170 hasAuthorship W1623367170A5001467466 @default.
• W1623367170 hasAuthorship W1623367170A5021491230 @default.
• W1623367170 hasAuthorship W1623367170A5052949728 @default.
• W1623367170 hasAuthorship W1623367170A5060444850 @default.
• W1623367170 hasAuthorship W1623367170A5081883425 @default.
• W1623367170 hasAuthorship W1623367170A5084226800 @default.
• W1623367170 hasAuthorship W1623367170A5086511870 @default.
• W1623367170 hasConcept C105702510 @default.
• W1623367170 hasConcept C106487976 @default.
• W1623367170 hasConcept C136229726 @default.
• W1623367170 hasConcept C142724271 @default.
• W1623367170 hasConcept C159470946 @default.
• W1623367170 hasConcept C185592680 @default.
• W1623367170 hasConcept C189165786 @default.
• W1623367170 hasConcept C197534660 @default.
• W1623367170 hasConcept C204787440 @default.
• W1623367170 hasConcept C2776164576 @default.
• W1623367170 hasConcept C2778232976 @default.
• W1623367170 hasConcept C2779335624 @default.
• W1623367170 hasConcept C2779794767 @default.
• W1623367170 hasConcept C2780550940 @default.
• W1623367170 hasConcept C2781013607 @default.
• W1623367170 hasConcept C2781403057 @default.
• W1623367170 hasConcept C3020332539 @default.

• next