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- W1623506302 abstract "Biomedical applications of low-energy (< 2nJ) near infrared (NIR) femtosecond laser pulses provided by compact, turn-key Ti:sapphire lasers are presented in this review. Applications include (i) ultrahigh resolution optical diagnostics (optical biopsies), (ii) gene therapy by optical targeted transfection of cells, and (iii) ultraprecise laser therapy (nanosurgery). The novel femtosecond laser system DermaInspec (JenLab GmbH) enables for the first time in vivo deep tissue imaging of intracellular compartments with submicron spatial and picosecond temporal resolution in patients with dermatological disorders. Using the system FemtOcut , intracellular surgery, optical gene transfer, and intraocular refractive surgery can be performed. The major process behind the diagnostical and therapeutical laser effects is non-resonant multiphoton absorption which results in two-photon autofluorescence and second harmonic generation at transient intensities of GW/cm2 as well as multiphoton ionization and plasma formation at TW/cm2 intensities, respectively.© (2004) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only." @default.
- W1623506302 created "2016-06-24" @default.
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- W1623506302 date "2004-06-01" @default.
- W1623506302 modified "2023-09-23" @default.
- W1623506302 title "Multiphoton tomography, transfection, and nanosurgery with <2-nJ, 80-MHz femtosecond laser pulses" @default.
- W1623506302 doi "https://doi.org/10.1117/12.528240" @default.
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