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- W1626820052 abstract "Publisher Summary Microtechniques have received widespread use in the crystallization of tRNA's, where only extremely small amounts of material are available. The protein to be crystallized is brought to the desired concentration in dilute buffer and a 10 μl droplet is placed in the well of a siliconed microculture slide. At any pH a preliminary test should be made to establish the concentration of salt, for example ammonium sulfate, at which the protein precipitates. This can be accomplished by adding strongly buffered saturated salt solutions to the 10 μl droplet with a 10 μl Hamilton syringe until the precipitation point is reached. Large crystals may sometimes be encouraged to grow by the use of a seeding solution. Such a solution is prepared by crushing a single crystal or group of crystals in a “stabilizing” solvent in which the crystal fragments will not redissolve. The suspension may then be centrifuged with a low speed clinical centrifuge to remove large fragments and diluted in the stabilizing solvent to minimize the concentration of nucleation sites. The sample to be crystallized is brought to a precipitant concentration slightly lower than that required to yield crystals without seeding and is then touched with a glass fiber which has been wetted with the seeding solution. This procedure frequently leads to the production of a few large crystals." @default.
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- W1626820052 date "1971-01-01" @default.
- W1626820052 modified "2023-09-25" @default.
- W1626820052 title "[25] Protein crystallization: Micro techniques involving vapor diffusion" @default.
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- W1626820052 doi "https://doi.org/10.1016/0076-6879(71)22027-9" @default.
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