FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W162686598> ?p ?o ?g. }

• W162686598 endingPage "489" @default.
• W162686598 startingPage "481" @default.
• W162686598 abstract "1. Phosphoribulokinase was purified 286-fold from extracts of autotrophically grown cells. 2. The enzyme had a molecular weight of 237000 and showed a pH optimum of 9.0 in both crude extracts and purified preparation. MgCl(2) was required for activity; full activation was obtained at 5mm-MgCl(2) and the K(m) was approx. 0.5mm. 3. The ATP-saturation curve was sigmoidal and the degree of positive co-operativity increased at higher MgCl(2) concentrations. The ATP-binding sites appeared to be non-interacting at low ribulose 5-phosphate concentrations. 4. Lineweaver-Burk plots for ribulose 5-phosphate showed abrupt transitions between apparently linear sections. The apparent K(m) and V(max.) values increased with increasing concentrations of ribulose phosphate. The transitions may be explained by a sequence of negative and positive co-operativity in the catalytic rate constants. 5. Phosphoribulokinase activity was inhibited by AMP and phosphoenolpyruvate and was activated by NADH. The presence of AMP or phosphoenolpyruvate increased s(0.5) (substrate concentration required for half-maximal velocity) for both ribulose 5-phosphate and ATP but V(max.) was not changed. The sigmoidicity of the ATP-saturation curve increased in the presence of AMP but was not affected by phosphoenolpyruvate. The transitions in the ribulose 5-phosphate-saturation curves were more abrupt in the presence of either inhibitor. NADH lowered the s(0.5) for both ribulose 5-phosphate and ATP. The activator did not affect the degree of positive co-operativity between ATP-binding sites, but the ribulose 5-phosphate-binding sites appeared to be non-interacting in its presence. 6. A sequence of positive and negative co-operativity in the interactions of AMP-binding sites was suggested by the Hill plots. In the presence of NADH (and phosphoenolpyruvate) the sensitivity to inhibition by AMP was less below a certain AMP concentration and increased above that concentration. 7. Examination of the interactions between ligands indicated that phosphoribulokinase can be regulated effectively by changes in effector concentrations similar to those reported to occur in vivo." @default.
• W162686598 created "2016-06-24" @default.
• W162686598 creator A5073173002 @default.
• W162686598 creator A5090693669 @default.
• W162686598 date "1974-06-01" @default.
• W162686598 modified "2023-09-23" @default.
• W162686598 title "Purification and regulatory properties of phosphoribulokinase from <i>Hydrogenomonas eutropha</i> H 16" @default.
• W162686598 cites W142391650 @default.
• W162686598 cites W1451443600 @default.
• W162686598 cites W1499814836 @default.
• W162686598 cites W1529479075 @default.
• W162686598 cites W1535190888 @default.
• W162686598 cites W1549484939 @default.
• W162686598 cites W1587995877 @default.
• W162686598 cites W1775749144 @default.
• W162686598 cites W1964045392 @default.
• W162686598 cites W1982571229 @default.
• W162686598 cites W2001651235 @default.
• W162686598 cites W2009652285 @default.
• W162686598 cites W2010678089 @default.
• W162686598 cites W2048931882 @default.
• W162686598 cites W2050120112 @default.
• W162686598 cites W2056116264 @default.
• W162686598 cites W2057699074 @default.
• W162686598 cites W2061194398 @default.
• W162686598 cites W2063074333 @default.
• W162686598 cites W2068624293 @default.
• W162686598 cites W2070127512 @default.
• W162686598 cites W2074581799 @default.
• W162686598 cites W2089138855 @default.
• W162686598 cites W2166456039 @default.
• W162686598 cites W2406890174 @default.
• W162686598 cites W77951017 @default.
• W162686598 doi "https://doi.org/10.1042/bj1390481" @default.
• W162686598 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1166312" @default.
• W162686598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4369092" @default.
• W162686598 hasPublicationYear "1974" @default.
• W162686598 type Work @default.
• W162686598 sameAs 162686598 @default.
• W162686598 citedByCount "33" @default.
• W162686598 countsByYear W1626865982022 @default.
• W162686598 crossrefType "journal-article" @default.
• W162686598 hasAuthorship W162686598A5073173002 @default.
• W162686598 hasAuthorship W162686598A5090693669 @default.
• W162686598 hasBestOaLocation W1626865982 @default.
• W162686598 hasConcept C104317684 @default.
• W162686598 hasConcept C114614502 @default.
• W162686598 hasConcept C143937172 @default.
• W162686598 hasConcept C181199279 @default.
• W162686598 hasConcept C185592680 @default.
• W162686598 hasConcept C2777132085 @default.
• W162686598 hasConcept C2779966244 @default.
• W162686598 hasConcept C28718788 @default.
• W162686598 hasConcept C33923547 @default.
• W162686598 hasConcept C50212798 @default.
• W162686598 hasConcept C55493867 @default.
• W162686598 hasConcept C71240020 @default.
• W162686598 hasConcept C86803240 @default.
• W162686598 hasConcept C88045685 @default.
• W162686598 hasConcept C9930424 @default.
• W162686598 hasConceptScore W162686598C104317684 @default.
• W162686598 hasConceptScore W162686598C114614502 @default.
• W162686598 hasConceptScore W162686598C143937172 @default.
• W162686598 hasConceptScore W162686598C181199279 @default.
• W162686598 hasConceptScore W162686598C185592680 @default.
• W162686598 hasConceptScore W162686598C2777132085 @default.
• W162686598 hasConceptScore W162686598C2779966244 @default.
• W162686598 hasConceptScore W162686598C28718788 @default.
• W162686598 hasConceptScore W162686598C33923547 @default.
• W162686598 hasConceptScore W162686598C50212798 @default.
• W162686598 hasConceptScore W162686598C55493867 @default.
• W162686598 hasConceptScore W162686598C71240020 @default.
• W162686598 hasConceptScore W162686598C86803240 @default.
• W162686598 hasConceptScore W162686598C88045685 @default.
• W162686598 hasConceptScore W162686598C9930424 @default.
• W162686598 hasIssue "3" @default.
• W162686598 hasLocation W1626865981 @default.
• W162686598 hasLocation W1626865982 @default.
• W162686598 hasLocation W1626865983 @default.
• W162686598 hasLocation W1626865984 @default.
• W162686598 hasOpenAccess W162686598 @default.
• W162686598 hasPrimaryLocation W1626865981 @default.
• W162686598 hasRelatedWork W162686598 @default.
• W162686598 hasRelatedWork W1909464692 @default.
• W162686598 hasRelatedWork W2022333369 @default.
• W162686598 hasRelatedWork W2049216461 @default.
• W162686598 hasRelatedWork W2073778632 @default.
• W162686598 hasRelatedWork W2113606128 @default.
• W162686598 hasRelatedWork W2148161241 @default.
• W162686598 hasRelatedWork W2169309436 @default.
• W162686598 hasRelatedWork W2309427866 @default.
• W162686598 hasRelatedWork W2530991042 @default.
• W162686598 hasVolume "139" @default.
• W162686598 isParatext "false" @default.
• W162686598 isRetracted "false" @default.
• W162686598 magId "162686598" @default.
• W162686598 workType "article" @default.