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- W1627876645 abstract "Water channel is arbitrary in a cell membrane because most channels that transport ions and polar solutes probably also transport some water. Water channel are those pathways that (1) make a substantial contribution to the net water permeability and (2) whose osmotic water permeability, Pf, is greater than its diffusional permeability, Pd. This chapter describes methods for the assay of water channels in red cells. The two systems that fulfill the criteria for water channels are red cells and vasopressin- stimulated toad bladder. Both diffusion and osmotic flow through a bilayer and through cell membranes are very rapid. Basic principles, design, and the application of continuous flow measurements of Pd to chemical reactions are described in the chapter. The method consists of introducing two rapidly flowing streams of reactants into a small mixing chamber. The chapter discusses a powerful nuclear magnetic resonance (NMR) technique for measuring Td (time constant). The technique is based on the fact that water protons orient their spins in a static magnetic field and that the application of an intense radio frequency reorients the spin." @default.
- W1627876645 created "2016-06-24" @default.
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- W1627876645 date "1986-01-01" @default.
- W1627876645 modified "2023-09-22" @default.
- W1627876645 title "[44] Water channels" @default.
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- W1627876645 doi "https://doi.org/10.1016/0076-6879(86)27047-0" @default.
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