FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W162883405> ?p ?o ?g. }

• W162883405 endingPage "62" @default.
• W162883405 startingPage "51" @default.
• W162883405 abstract "Burkholderia pseudomallei is a bacterium that causes a disease known as melioidosis. Infections of B. pseudomallei appear in several organs including acute septicemia which showed high mortality rate. The rapid and high efficient diagnostic test may reduce the mortality rate of melioidosis patients. We performed conventional PCR using LPS primers and real-time PCR using 16S rDNA primers for detection of B. pseudomallei in clinical blood specimens. Bacterial culture was used as a gold standard. Blood specimens from 32 suspected bacterial septicemia patients were obtained from admitted patients in Khon Kaen Hospital and other hospitals in the region. These consist of the patients with 19 B. pseudomallei and 13 other bacterial infections including 1 Burkholderia cepacia , 1 Escherichia coli , 4 Klebsiella pneumoniae, 1 Enterobacter species, 3 Staphylococcus aureus , 1 Streptococcus pneumoniae and 2 group A Streptococcus . The sensitivity, specificity, positive and negative predictive values were determined. Conventional PCR showed low sensitivity of 37 % (95% CI:15-59) and high specificity of 92 % (95% CI:78-100) with 88 % (95% CI: 65-100) and 50 % (95% CI:30-70) of positive predictive value (PPV) and negative predictive value (NPV), respectively. In contrast, a real-time PCR and using of combination test showed higher sensitivity (63 %, 95% CI: 41-85) and lower specificity (69 %, 95% CI: 44-94) with 75 % (95% CI:54-96) and 56 % (95% CI:32-81) of PPV and NPV, respectively. In 5 melioidosis patients who have died, both PCR methods showed rising of sensitivity [80 % (95 % CI: 45-100) and 100 % (95% CI: 100-100)], respectively. The lower detection limit of B. pseudomallei by conventional PCR was 10 3 cfu/ml. The conventional PCR and real-time PCR could detect B. pseudomallei DNA of 10 pg and 50 fg per PCR reaction, respectively. The higher sensitivity of real-time PCR may be useful for early screening test, whereas the conventional PCR with higher specificity and PPV may be used as a confirmatory test for diagnosis of B. pseudomallei. Therefore, the two assays may be used in combination as rapid molecular diagnostic for melioidosis which might lead to a reduction in the mortality rate of melioidosis patients." @default.
• W162883405 created "2016-06-24" @default.
• W162883405 creator A5025181325 @default.
• W162883405 creator A5037134593 @default.
• W162883405 creator A5041851954 @default.
• W162883405 creator A5066128405 @default.
• W162883405 creator A5088627834 @default.
• W162883405 date "2010-04-11" @default.
• W162883405 modified "2023-09-23" @default.
• W162883405 title "Detection of Burkholria pseudomallei DNA in whole blood samples of bacterial septicemia patients using conventional PCR and real-time PCR" @default.
• W162883405 cites W1938068180 @default.
• W162883405 cites W1972970671 @default.
• W162883405 cites W1986369193 @default.
• W162883405 cites W1993928378 @default.
• W162883405 cites W2010231122 @default.
• W162883405 cites W2015241243 @default.
• W162883405 cites W2016473722 @default.
• W162883405 cites W2022177582 @default.
• W162883405 cites W2049336537 @default.
• W162883405 cites W2082873713 @default.
• W162883405 cites W2088284604 @default.
• W162883405 cites W2098694771 @default.
• W162883405 cites W2099084254 @default.
• W162883405 cites W2101891378 @default.
• W162883405 cites W2102846951 @default.
• W162883405 cites W2104695011 @default.
• W162883405 cites W2105753785 @default.
• W162883405 cites W2110977811 @default.
• W162883405 cites W2112760723 @default.
• W162883405 cites W2116832454 @default.
• W162883405 cites W2123403764 @default.
• W162883405 cites W2135261732 @default.
• W162883405 cites W2135485869 @default.
• W162883405 cites W2137304822 @default.
• W162883405 cites W2150238431 @default.
• W162883405 cites W2196553987 @default.
• W162883405 hasPublicationYear "2010" @default.
• W162883405 type Work @default.
• W162883405 sameAs 162883405 @default.
• W162883405 citedByCount "0" @default.
• W162883405 crossrefType "journal-article" @default.
• W162883405 hasAuthorship W162883405A5025181325 @default.
• W162883405 hasAuthorship W162883405A5037134593 @default.
• W162883405 hasAuthorship W162883405A5041851954 @default.
• W162883405 hasAuthorship W162883405A5066128405 @default.
• W162883405 hasAuthorship W162883405A5088627834 @default.
• W162883405 hasConcept C104317684 @default.
• W162883405 hasConcept C126322002 @default.
• W162883405 hasConcept C2777058267 @default.
• W162883405 hasConcept C2779110727 @default.
• W162883405 hasConcept C2779473907 @default.
• W162883405 hasConcept C2779489039 @default.
• W162883405 hasConcept C2779775996 @default.
• W162883405 hasConcept C2781253189 @default.
• W162883405 hasConcept C40993552 @default.
• W162883405 hasConcept C49105822 @default.
• W162883405 hasConcept C501593827 @default.
• W162883405 hasConcept C523546767 @default.
• W162883405 hasConcept C54355233 @default.
• W162883405 hasConcept C547475151 @default.
• W162883405 hasConcept C55493867 @default.
• W162883405 hasConcept C71924100 @default.
• W162883405 hasConcept C86803240 @default.
• W162883405 hasConcept C89423630 @default.
• W162883405 hasConcept C90924648 @default.
• W162883405 hasConceptScore W162883405C104317684 @default.
• W162883405 hasConceptScore W162883405C126322002 @default.
• W162883405 hasConceptScore W162883405C2777058267 @default.
• W162883405 hasConceptScore W162883405C2779110727 @default.
• W162883405 hasConceptScore W162883405C2779473907 @default.
• W162883405 hasConceptScore W162883405C2779489039 @default.
• W162883405 hasConceptScore W162883405C2779775996 @default.
• W162883405 hasConceptScore W162883405C2781253189 @default.
• W162883405 hasConceptScore W162883405C40993552 @default.
• W162883405 hasConceptScore W162883405C49105822 @default.
• W162883405 hasConceptScore W162883405C501593827 @default.
• W162883405 hasConceptScore W162883405C523546767 @default.
• W162883405 hasConceptScore W162883405C54355233 @default.
• W162883405 hasConceptScore W162883405C547475151 @default.
• W162883405 hasConceptScore W162883405C55493867 @default.
• W162883405 hasConceptScore W162883405C71924100 @default.
• W162883405 hasConceptScore W162883405C86803240 @default.
• W162883405 hasConceptScore W162883405C89423630 @default.
• W162883405 hasConceptScore W162883405C90924648 @default.
• W162883405 hasIssue "1" @default.
• W162883405 hasLocation W1628834051 @default.
• W162883405 hasOpenAccess W162883405 @default.
• W162883405 hasPrimaryLocation W1628834051 @default.
• W162883405 hasRelatedWork W1520353793 @default.
• W162883405 hasRelatedWork W1896031507 @default.
• W162883405 hasRelatedWork W2011509506 @default.
• W162883405 hasRelatedWork W2093867021 @default.
• W162883405 hasRelatedWork W2109142392 @default.
• W162883405 hasRelatedWork W2111164110 @default.
• W162883405 hasRelatedWork W2149392734 @default.
• W162883405 hasRelatedWork W2259108794 @default.
• W162883405 hasRelatedWork W2265844719 @default.
• W162883405 hasRelatedWork W2392211478 @default.

• next