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- W1628976724 abstract "The volume of agar medium in a Petri dish was found to be related to T-mycoplasmal colonial size and morphology. Increasing the volume of agar was associated with an increase in colony size and with a change from tiny granular colonies to larger colonies with a “fried egg” appearance. lnoculum size was inversely related to colony size. Hepes buffer had little effect on colony size under the conditions utilized in these studies. 1 -mycoplasmas, first isolated by Shepard,l were designated T (for Tiny) strains because they produced small granular colonies on agar rather than the large “fried egg” colonies produced by most other mycoplasmal species. Colony size is thus one of the characteristics of the T-mycoplasmas in addition to their ability to metabolize urea, their resistance to lincomycin, and their susceptibility to erythromycin.‘-” Although it has been reported that T-mycoplasmal colonial size and morphology is influenced by PH,~, 5 urea concentratioq7 and the atmosphere in which the plates are incubated, 3* 4 Shepard has shown that even under the most favorable of conditions colony size rarely exceeds 25 pm in diameter.* More recently, Manchee and Taylor-Robinson8 have reported that the addition of Hepes (N-2hydroxyethylpiperazine-N-2-ethansulfonic" @default.
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- W1628976724 date "1974-11-01" @default.
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- W1628976724 title "Effect of agar volume, inoculum size, and Hepes buffer on the size of T-mycoplasmal colonies." @default.
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