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• W1631467609 abstract "Publisher Summary This chapter describes the process of measuring changes in platelet cyclic adenosine monophosphate (AMP) in vitro and in vivo by prelabeling techniques. It is possible to use the formation of labeled cyclic AMP in platelets as a method of detecting and assaying compounds that activate platelet adenylate cyclases such as prostacyclin (PGI 2 ). In principle, this is no different from methods that use the associated inhibition of platelet function as the basis of an assay. However, by including an inhibitor of cyclic AMP phosphodiesterase such as 3-isobutyl-1-methylxanthine (IBMX), the accumulation of labeled cyclic AMP is greatly enhanced and assay sensitivity is increased. This is not so easily achieved in assays based on platelet function because high concentrations of IBMX alone block platelet responses. These types of assays must include some means of identifying the compound responsible for increases in platelet cyclic AMP. Prelabeling techniques have been extended for the study of the relationship between changes in platelet cyclic AMP and platelet responsiveness in whole blood from rabbits transfused with labeled platelets." @default.
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• W1631467609 date "1989-01-01" @default.
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• W1631467609 title "[37] Measurement of changes in platelet cyclic AMP in vitro and in vivo by prelabeling techniques: Application to the detection and assay of circulating PGI2" @default.
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• W1631467609 doi "https://doi.org/10.1016/0076-6879(89)69081-7" @default.
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